Transgenic Expression of Arabidopsis ELONGATION FACTOR-TU RECEPTOR (AtEFR) Gene in Banana Enhances Resistance Against Xanthomonas campestris pv. musacearum

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dc.contributor.authorAdero, Mark
dc.contributor.authorTripathi, Jaindra Nath
dc.contributor.authorOduor, Richard
dc.contributor.authorZipfel, Cyril
dc.contributor.authorTripathi, Leena
dc.date.accessioned2023-09-06T09:10:16Z
dc.date.available2023-09-06T09:10:16Z
dc.date.issued2023
dc.descriptionArticleen_US
dc.description.abstractBanana Xanthomonas wilt (BXW) caused by Xanthomonas campestris pv. musacearum (Xcm) is a severe bacterial disease affecting banana production in East and Central Africa, where banana is cultivated as a staple crop. Classical breeding of banana is challenging because the crop is clonally propagated and has limited genetic diversity. Thus, genetic engineering serves as a viable alternative for banana improvement. Studies have shown that transfer of the elongation factor Tu receptor gene (AtEFR) from Arabidopsis thaliana to other plant species can enhance resistance against bacterial diseases. However, AtEFR activity in banana and its efficacy against Xcm has not been demonstrated. In this study, transgenic events of banana (Musa acuminata) cultivar dwarf Cavendish expressing the AtEFR gene were generated and evaluated for resistance against Xcm under greenhouse conditions. The transgenic banana events were responsive to the EF-Tu-derived elf18 peptide and exhibited enhanced resistance to BXW disease compared to non-transgenic control plants. This study suggests that the functionality of AtEFR is retained in banana with the potential of enhancing resistance to BXW under field conditionsen_US
dc.description.sponsorship2 Blades Foundation Gatsby Charitable Foundation United States Agency for International Development (USAID)en_US
dc.identifier.citationAdero M, Tripathi JN, Oduor R, Zipfel C, Tripathi L (2023) Transgenic expression of Arabidopsis ELONGATION FACTOR-TU RECEPTOR (AtEFR) gene in banana enhances resistance against Xanthomonas campestris pv. musacearum. PLoS ONE 18(9): e0290884. https:// doi.org/10.1371/journal.pone.0290884en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0290884
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/26900
dc.language.isoenen_US
dc.publisherplos oneen_US
dc.titleTransgenic Expression of Arabidopsis ELONGATION FACTOR-TU RECEPTOR (AtEFR) Gene in Banana Enhances Resistance Against Xanthomonas campestris pv. musacearumen_US
dc.typeArticleen_US
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