Occurrence of Stromal Derived Factor-1 Polymorphism In Kenyan Population
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Date
2012
Authors
Gicheru, M. M.
Ongonda, John K
Khamadi, Samoel A
Journal Title
Journal ISSN
Volume Title
Publisher
WebmedCentral
Abstract
Genetic polymorphism in chemokine receptors and
coreceptor genes influences susceptibility to HIV-1
infection and disease progression. The mutated
Stromal Derived Factor-1 3A`/3A` (SDF-1 3’A)
competes with the virus for the coreceptor site
Cystein-X-cystein receptor 4 (CXCR4) on the CD4+
T-cells therefore down-regulating evolution of
non-syncytial to syncytial induction during HIV-1
progression. Two hundred whole blood samples were
collected from eight provinces of Kenya and analysed
at the Kenya Medical Research Institute in Nairobi.
Detection of SDF-1 gene polymorphism was done by
extraction of proviral DNA from whole blood and the
SDF-1 target gene was amplified by polymerase chain
reaction using gene-specific primers. The different
SDF-1 gene polymorphisms were detected by
Restriction Fragment Length Polymorphism and gel
electrophoresis. Chi-square test was used to test for
the significance in the distributions of these
polymorphism. The relationship between the
frequency of SDF-1 mutation and HIV prevalence was
analysed using Pearson’s product moment of
correlation coefficient (r). This study showed the
presence of the stromal derived factor-1 polymorphism
in Kenyan population with an average of 6.6% for the
double mutant, 20.7% for heterozygous and 73.7% for
the wild type. There was no correlation between the
HIV-1 prevalence and the SDF-1 distribution in Kenya.
These results will form a foundation for further
research in Kenya given that double mutants had been
found to resist HIV-1 infection or show good response
to anti-retroviral drugs. The researchers can therefore
incorporate genetics in the treatment of HIV-1.
Description
Keywords
Stromal derived factor-1, Mutations, Coreceptors, HIV, PCR, RFLPRFLP
Citation
IMMUNOLOGY 2012;3(4):WMC001556