Occurrence of Stromal Derived Factor-1 Polymorphism In Kenyan Population
Gicheru, M. M.
Ongonda, John K
Khamadi, Samoel A
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Genetic polymorphism in chemokine receptors and coreceptor genes influences susceptibility to HIV-1 infection and disease progression. The mutated Stromal Derived Factor-1 3A`/3A` (SDF-1 3’A) competes with the virus for the coreceptor site Cystein-X-cystein receptor 4 (CXCR4) on the CD4+ T-cells therefore down-regulating evolution of non-syncytial to syncytial induction during HIV-1 progression. Two hundred whole blood samples were collected from eight provinces of Kenya and analysed at the Kenya Medical Research Institute in Nairobi. Detection of SDF-1 gene polymorphism was done by extraction of proviral DNA from whole blood and the SDF-1 target gene was amplified by polymerase chain reaction using gene-specific primers. The different SDF-1 gene polymorphisms were detected by Restriction Fragment Length Polymorphism and gel electrophoresis. Chi-square test was used to test for the significance in the distributions of these polymorphism. The relationship between the frequency of SDF-1 mutation and HIV prevalence was analysed using Pearson’s product moment of correlation coefficient (r). This study showed the presence of the stromal derived factor-1 polymorphism in Kenyan population with an average of 6.6% for the double mutant, 20.7% for heterozygous and 73.7% for the wild type. There was no correlation between the HIV-1 prevalence and the SDF-1 distribution in Kenya. These results will form a foundation for further research in Kenya given that double mutants had been found to resist HIV-1 infection or show good response to anti-retroviral drugs. The researchers can therefore incorporate genetics in the treatment of HIV-1.