Estimates of outcrossing rates in Moringa oleifera using Amplified fragment length polymorphism (AFLP)

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dc.contributor.authorMuluvi, G. M.
dc.contributor.authorSprent, J.I.
dc.contributor.authorOdee, David W.
dc.contributor.authorPowell, W
dc.date.accessioned2012-10-18T11:50:17Z
dc.date.available2012-10-18T11:50:17Z
dc.date.issued2004-02
dc.descriptionPublisher version (African Journals Online available at www.ajol.info/index.php/ajb/article/view/14932/58843en_US
dc.description.abstractestimates of the outcrosing rates are often required for planning breeding programmes, conservation and management of tropical trees. However, although Moringa oleifera is adapted to a mixed mating system, the proportion of selfing has not been previously estimated. The current work therefore, shows the use of AFLP markers in a mating system study of M. oleifera seed orchard. Data revealed a mixed mating system with a multilocus outcrossing rate (tm) of 0.74. It further demonstrated that AFLP markers, though dominant with a lower information content than co-dominant markers are adequate for the study of the mating system in plant populations. The 26% selfing observed in M. oleifera can lead to overestimation of the proportion of additive genetic variance and appropriate adjustments are therefore required. However, the presence of selfing as well as early sexual maturity (6 months to 1 year) in M. oleifera provides an opportunity for developing inbred lines and hybridisation. Additionally, in designing M. oleifera seed orchards, randomisation and minimum distance between related individuals need to be worked out to maximise cross-fertilisation among unrelated clones and minimise selfing or mating among related ramets.en_US
dc.description.sponsorshipEU contract ERBTS38-CT94-0309.en_US
dc.identifier.citationAfrican Journal of Biotechnology Vol. 3 (2), pp. 146-151, February 2004en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/5755
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectMoringa oleiferaen_US
dc.subjectmating systemen_US
dc.subjectoutcrossing ratesen_US
dc.subjectamplified fragment length polymorphismen_US
dc.titleEstimates of outcrossing rates in Moringa oleifera using Amplified fragment length polymorphism (AFLP)en_US
dc.typeArticleen_US
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