Tryptophan metabolism in vitamin B6-deficient mice

Simple item page
dc.contributor.authorNjagi, E.N.M.
dc.contributor.authorDanielian, P. S.
dc.contributor.authorBender, D. A.
dc.date.accessioned2014-06-04T13:59:05Z
dc.date.available2014-06-04T13:59:05Z
dc.date.issued1990-01
dc.descriptionDOI: http://dx.doi.org/10.1079/BJN19900089en_US
dc.description.abstractVitamin B6 deficiency was induced in mice by maintenance for 4 weeks on a vitamin B6-free diet. Tryptophan metabolism was assessed by determining the urinary excretion of tryptophan metabolites, the metabolism of [14C]tryptophan in vivo and the formation of tryptophan and niacin metabolites by isolated hepatocytes. The vitamin B6-deficient animals excreted more xanthurenic acid and 3-hydroxykynurenine, and less of the niacin metabolites N1-methyl nicotinamide and methyl-2-pyridone-4-carboxamide, than did control animals maintained on the same diet supplemented with 5 mg vitamin B6/kg. After intraperitoneal injection of [14C]tryptophan, vitamin B6-deficient mice showed lower liberation of14CO2 from [methylene-14C]tryptophan and [U-14C]tryptophan than did controls, indicating impairment of kynureninase (EC 3.7.1.3) activity. There was no difference between the two groups of animals in the metabolism of [ring-2-14C]tryptophan. Hepatocytes isolated from the vitamin B6-deficient animals formed more 3-hydroxykynurenine and xanthurenic acid than did cells from control animals, but also formed more NADP and free niacin.en_US
dc.identifier.citationBritish Journal of Nutrition / Volume 63 / Issue 01 / January 1990, pp 27-36en_US
dc.identifier.issn0007-1145
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/9790
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.subjectTryptophan metabolismen_US
dc.subjectVitamin B6 deficiencyen_US
dc.subjectMiceen_US
dc.titleTryptophan metabolism in vitamin B6-deficient miceen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Tryptophan metabolism in vitamin B,-deficient mice.pdf
Size:
584.58 KB
Format:
Adobe Portable Document Format
Description:
Full Text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Biochemistry and Biotechnology