Occurrence of a Novel Strain of Moroccan Watermelon Mosaic Virus Infecting Pumpkins in Kenya

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dc.contributor.authorMumo, Naomi Nzilani
dc.contributor.authorAteka, Elijah Miinda
dc.contributor.authorMamati, Edward George
dc.contributor.authorRimberia, Fredah K.
dc.contributor.authorAsudi, George Ochieng’
dc.contributor.authorMachuka, Eunice
dc.contributor.authorNjuguna, Joyce Njoki
dc.contributor.authorStomeo, Francesca
dc.contributor.authorPelle, Roger
dc.date.accessioned2023-07-25T08:29:47Z
dc.date.available2023-07-25T08:29:47Z
dc.date.issued2022
dc.descriptionarticleen_US
dc.description.abstractThe Potyvirus Moroccan watermelon mosaic virus (MWMV) naturally infects and severely threatens production of cucurbits and papaya. In this study, we identified and characterized MWMV isolated from pumpkin (Cucurbita moschata) intercropped with MWMV-infected papaya plants through next-generation sequencing (NGS) and Sanger sequencing approaches. Complete MWMV genome sequences were obtained from two pumpkin samples through NGS and validated using Sanger sequencing. The isolates shared 83.4 to 83.7% nucleotide (nt) and 92.3 to 95.1% amino acid (aa) sequence identities in the coat protein and 79.5 to 79.9% nt and 89.2 to 89.7% aa identities in the polyprotein with papaya isolates of MWMV. Phylogenetic analysis using complete polyprotein nt sequences revealed the clustering of both pumpkin isolates of MWMV with corresponding sequences of cucurbit isolates of the virus from other parts of Africa and the Mediterranean regions, distinct from a clade formed by papaya isolates. Through sap inoculation, a pumpkin isolate of MWMV was pathogenic on zucchini (Cucurbita pepo), watermelon (Citrullus lanatus), and cucumber (Cucumis sativus) but not on papaya. Conversely, the papaya isolate of MWMV was nonpathogenic on pumpkin, watermelon, and cucumber, but it infected zucchini. The results suggest the occurrence of two strains of MWMV in Kenya having different biological characteristics associated with the host specificity.en_US
dc.description.sponsorshipAfrica Biosciences Challenge Fund (ABCF)en_US
dc.identifier.citationMumo, N. N., Ateka, E. M., Mamati, E. G., Rimberia, F. K., Asudi, G. O., Machuka, E., ... & Pelle, R. (2022). Occurrence of a Novel Strain of Moroccan Watermelon Mosaic Virus Infecting Pumpkins in Kenya. Plant Disease, 106(1), 39-45.en_US
dc.identifier.urihttps://doi.org/10.1094/PDIS-02-21-0359-RE
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/26388
dc.language.isoenen_US
dc.subjectdisease managementen_US
dc.subjecttree fruitsen_US
dc.subjectvirusesen_US
dc.subjectviroidsen_US
dc.titleOccurrence of a Novel Strain of Moroccan Watermelon Mosaic Virus Infecting Pumpkins in Kenyaen_US
dc.typeArticleen_US
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