In Vitro Regeneration Protocol of Kenyan Adapted Groundnut (Arachis hypogaea L.) Genotypes using Cotyledonary Node Explants
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Date
2019
Authors
Limbua, Purity Gacheri
Ngugi, Mathew Piero
Oduor, Richard Okoth
Journal Title
Journal ISSN
Volume Title
Publisher
Longdom
Abstract
A reproducible regeneration protocol for ICGV 12991, CG 7 and Red Valencia groundnut genotypes using
Cotyledonary Node explants has been optimized. The effect of different BAP concentrations combined with either
2,4-D or TDZ was tested to determine optimum conditions for high shoot induction. Different BAP concentrations
were tested to determine an optimum concentration for shoot elongation. Different NAA concentrations were
similarly evaluated to determine the best concentration for rooting. Media containing combination of 5 mg/L BAP and
1 mg/L TDZ was the best concentration for shoot induction while media containing BAP at 5 mg/L was the best for
elongation of shoots. NAA concentration of 1 mg/L gave the highest number of plants with roots. This works
provides a very good protocol which will be beneficial during groundnut tissue culture as well as genetic
transformation of groundnuts.
Description
A research article published in Journal of Plant Biochemistry &
Physiology
Keywords
Cotyledonary node explants, Optimized protocol, Kenyan groundnut genotypes
Citation
Journal of Plant Biochemistry & Physiology. Vol 7 Iss 1