In vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in Kenyan women

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dc.contributor.authorMatu, Martin N.
dc.contributor.authorOrinda, G. O.
dc.contributor.authorNjagi, E.N.M.
dc.contributor.authorCohen, Craig R.
dc.contributor.authorBukusi, Elizabeth A.
dc.date.accessioned2012-11-02T12:04:59Z
dc.date.available2012-11-02T12:04:59Z
dc.date.issued2010-06
dc.description.abstractLactobacilli have been shown to inhibit in vitro growth of many pathogens and have been used as probiotics to treat a broad range of gastrointestinal and/or vaginal disorders. We sought to determine the in vitro inhibitory potential of lactobacilli of vaginal origin to some bacteria associated with bacterial vaginosis (BV), to characterize the inhibitory substances produced by these lactobacilli and to assess H2O2 production. Vaginal specimens were obtained by swabbing the lateral vaginal walls from 107 women two months following BV treatment. One hundred and fifty eight Lactobacillus spp. were isolated in 82 of the 107 women. Lactobacillus jensenii was the predominant strain isolated among these women (29/158; 18.4%). Among 158 culture supernatants tested for antibacterial activity against BV-associated bacteria, none inhibited the growth of Bacteroides fragilis while 23% (37/158), 28% (45/158) and 29% (46/158) inhibited the growth of Prevotella bivia, Gardnerella vaginalis and Mobiluncus spp. respectively. The lactobacilli produced supernatants with a pH range between 2.62 and 6.71; the highly acidic (pH 2–3.99) supernatants were more inhibitory to the indicator strains. There was significant reduction in the mean zones of inhibition following chemical and physical treatment of the supernatants (p = 0.0025). Acid, bacteriocins and H2O2 demonstrated potential for antagonism of the bacterial pathogens. These substances may augment each other rather that each working independently on the pathogens.en_US
dc.identifier.citationAnaerobe Volume 16, Issue 3, June 2010, Pages 210–215en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/5929
dc.language.isoenen_US
dc.publisherScience Directen_US
dc.subjectVaginal lactobacillien_US
dc.subjectBacterial vaginosisen_US
dc.subjectIndicator bacteriaen_US
dc.subjectAntagonistic activityen_US
dc.subjectHydrogen peroxideen_US
dc.titleIn vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in Kenyan womenen_US
dc.typeArticleen_US
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