In Vivo Ameliorative Effects of Vitamin E against Hydralazine-Induced Lupus
Loading...
Date
2023
Authors
Githaiga, Fiona Muthoni
Omwenga, George Isanda
Ngugi, Mathew Piero
Journal Title
Journal ISSN
Volume Title
Publisher
BMJ
Abstract
ABSTRACT
Objective In this study, we investigated the in vivo
ameliorative effects of vitamin E in a hydralazine-induced
lupus model, which closely resembles SLE in humans. We
aim to shed light on its potential as a therapeutic agent for
managing SLE.
Methods Forty BALB/c mice were used in this study.
Hydralazine hydrochloride was orally administered in a
concentration of 25mg/kg to the five mice groups once
weekly for a period of 5weeks to induce a lupus-like
condition. The untreated group was the normal control
group. To confirm the development of lupus, an ANA
test was conducted. After the mice tested positive for
ANA, drug treatments commenced. The negative control
group did not receive any drug treatment. The treatments
included prednisolone, methotrexate and vitamin E, all
administered at a concentration of 25mg/kg, with a higher
dose of vitamin E (50mg/kg) also administered.
Results Notably, on day 35, after drug treatment, we
observed that mice that received vitamin E at a dosage
of 50mg/kg (3.01±0.100) had a slight decrease in
lymphocyte hydrogen peroxide radicals when compared
with the group receiving 25mg/kg of vitamin E
(3.30±0.100) (p<0.05). This finding suggests that the
scavenging potential of vitamin E is dose dependent.
Conclusion This study suggests that vitamin E
supplementation, especially at a higher dose (50mg/
kg), holds promise in ameliorating lupus-like conditions.
These findings warrant further exploration and may offer
a potential avenue for improving the disease status of
patients experiencing SLE.
Description
Article
Keywords
vivo ameliorative, vitamin E, hydralazine-induced lupus
Citation
Githaiga FM, Omwenga GI, Ngugi MP. In vivo ameliorative effects of vitamin E against hydralazine-induced lupus. Lupus Science & Medicine 2023;10:e001033. doi:10.1136/ lupus-2023-001033