The Cell Membrane of a Novel Rhizobium phaseoli Strain Is the Crucial Target for Aluminium Toxicity and Tolerance

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dc.contributor.authorWekesa, Clabe
dc.contributor.authorMuoma, John O.
dc.contributor.authorReichelt, Michael
dc.contributor.authorAsudi, George O.
dc.contributor.authorFurch, Alexandra C. U.
dc.contributor.authorOelmüller, Ralf
dc.date.accessioned2023-07-25T06:09:41Z
dc.date.available2023-07-25T06:09:41Z
dc.date.issued2022-03
dc.descriptionarticleen_US
dc.description.abstractSoils with low pH and high aluminium (Al) contamination restrict common bean production, mainly due to adverse effects on rhizobia. We isolated a novel rhizobium strain, B3, from Kenyan soil which is more tolerant to Al stress than the widely used commercial strain CIAT899. B3 was resistant to 50 M Al and recovered from 100 M Al stress, while CIAT899 did not. Calcein labeling showed that less Al binds to the B3 membranes and less ATP and mScarlet-1 protein, a cytoplasmic marker, leaked out of B3 than CIAT899 cells in Al-containing media. Expression profiles showed that the primary targets of Al are genes involved in membrane biogenesis, metal ions binding and transport, carbohydrate, and amino acid metabolism and transport. The identified differentially expressed genes suggested that the intracellular -aminobutyric acid (GABA), glutathione (GSH), and amino acid levels, as well as the amount of the extracellular exopolysaccharide (EPS), might change during Al stress. Altered EPS levels could also influence biofilm formation. Therefore, these parameters were investigated in more detail. The GABA levels, extracellular EPS production, and biofilm formation increased, while GSH and amino acid level decreased. In conclusion, our comparative analysis identified genes that respond to Al stress in R. phaseoli. It appears that a large portion of the identified genes code for proteins stabilizing the plasma membrane. These genes might be helpful for future studies investigating the molecular basis of Al tolerance and the characterization of candidate rhizobial isolates that perform better in Al-contaminated soils than commercial strains.en_US
dc.description.sponsorshipGermany Academic Exchange Service (DAAD) Friedrich Schiller University of Jena National Research Fund (NRF) Kenyaen_US
dc.identifier.citationWekesa, C.; Muoma, J.O.; Reichelt, M.; Asudi, G.O.; Furch, A.C.U.; Oelmüller, R. The Cell Membrane of a Novel Rhizobium phaseoli Strain Is the Crucial Target for Aluminium Toxicity and Tolerance. Cells 2022, 11, 873. https://doi.org/ 10.3390/cells11050873en_US
dc.identifier.urihttps://doi.org/ 10.3390/cells11050873
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/26380
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectaluminium toxicityen_US
dc.subjectRNA-Seqen_US
dc.subjectcommon beanen_US
dc.subjectRhizobium phaseolien_US
dc.subjectgene expressionen_US
dc.subjectaluminum toleranceen_US
dc.titleThe Cell Membrane of a Novel Rhizobium phaseoli Strain Is the Crucial Target for Aluminium Toxicity and Toleranceen_US
dc.typeArticleen_US
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