Microbial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopia

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dc.contributor.authorDesta, Adey Feleke
dc.contributor.authorAssefa, Fassil
dc.contributor.authorLeta, Seyoum
dc.contributor.authorStomeo, Francesca
dc.contributor.authorWamalwa, Mark
dc.contributor.authorNjahira, Moses
dc.contributor.authorAppolinaire, Djikeng
dc.date.accessioned2021-06-23T09:07:25Z
dc.date.available2021-06-23T09:07:25Z
dc.date.issued2014
dc.descriptionResearch Articleen_US
dc.description.abstractA culture-independent approach was used to elucidate the microbial diversity and structure in the anaerobic-aerobic reactors integrated with a constructed wetland for the treatment of tannery wastewater in Modjo town, Ethiopia. The system has been running with removal efficiencies ranging from 94%–96% for COD, 91%–100% for SO42- and S2-, 92%–94% for BOD, 56%–82% for total Nitrogen and 2%–90% for NH3-N. 16S rRNA gene clone libraries were constructed and microbial community assemblies were determined by analysis of a total of 801 unique clone sequences from all the sites. Operational Taxonomic Unit (OTU) - based analysis of the sequences revealed highly diverse communities in each of the reactors and the constructed wetland. A total of 32 phylotypes were identified with the dominant members affiliated to Clostridia (33%), Betaproteobacteria (10%), Bacteroidia (10%), Deltaproteobacteria (9%) and Gammaproteobacteria (6%). Sequences affiliated to the class Clostridia were the most abundant across all sites. The 801 sequences were assigned to 255 OTUs, of which 3 OTUs were shared among the clone libraries from all sites. The shared OTUs comprised 80 sequences belonging to Clostridiales Family XIII Incertae Sedis, Bacteroidetes and unclassified bacterial group. Significantly different communities were harbored by the anaerobic, aerobic and rhizosphere sites of the constructed wetland. Numerous representative genera of the dominant bacterial classes obtained from the different sample sites of the integrated system have been implicated in the removal of various carboncontaining pollutants of natural and synthetic origins. To our knowledge, this is theen_US
dc.description.sponsorshipSwedish International Development cooperation Agency (SIDA) via the East African Regional Programme and Research Network for Biotechnology, Biosafety and Policy Development (BIO-EARN) and Syngenta Foundation for Sustainable Agriculture (SFSA) via the biosciences Eastern and Central Africa (BecA)en_US
dc.identifier.citationDesta AF, Assefa F, Leta S, Stomeo F, Wamalwa M, et al. (2014) Microbial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopiaen_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/22406
dc.language.isoenen_US
dc.publisherPLOS ONEen_US
dc.titleMicrobial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopiaen_US
dc.typeArticleen_US
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