Detection of Anaplasma Antibodies in Wildlife and Domestic Species in Wildlife-Livestock Interface Areas of Kenya by Major Surface Protein 5 Competitive Inhibition Enzyme-Linked Immunosorbent Assay

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dc.contributor.authorNgeranwa, J.J.N
dc.contributor.authorShompole, S.P
dc.contributor.authorVenter, E.H
dc.contributor.authorWambugu, A
dc.contributor.authorCrafford, J.E
dc.contributor.authorPenzhorn, B.L
dc.date.accessioned2021-06-17T08:03:01Z
dc.date.available2021-06-17T08:03:01Z
dc.date.issued2008
dc.descriptionResearch Article.en_US
dc.description.abstractThe seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson’s gazelle, Grant’s gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife/livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers. Keywords: Anaplasma, CI-ELISA, Kenya, seroprevalence, wildlife-livestock interfaceen_US
dc.description.sponsorshipNetherlands Government, through the Wildlife Disease Research Project,en_US
dc.identifier.citationNgeranwa, J. J. N., Shompole, S. P., Venter, E. H., Wambugu, A., Crafford, J. E., & Penzhorn, B. L. (2008). Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay. Onderstepoort Journal of Veterinary Research, 75(3), 199-205.en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/22359
dc.language.isoenen_US
dc.publisherOnderstepoort Journal of Veterinary Researchen_US
dc.subjectAnaplasmaen_US
dc.subjectCI-ELISAen_US
dc.subjectKenyaen_US
dc.subjectseroprevalenceen_US
dc.subjectwildlife-livestock interfaceen_US
dc.titleDetection of Anaplasma Antibodies in Wildlife and Domestic Species in Wildlife-Livestock Interface Areas of Kenya by Major Surface Protein 5 Competitive Inhibition Enzyme-Linked Immunosorbent Assayen_US
dc.typeArticleen_US
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