Dicamba growth regulator promotes genotype independent somatic embryogenesis from immature zygotic embryos of tropical maize inbred lines.

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dc.contributor.authorAkoyi, J.
dc.contributor.authorMgutu, A. J.
dc.contributor.authorMachuka, Jesse
dc.contributor.authorLijsebettens, M.V.
dc.contributor.authorTaracha, C.
dc.contributor.authorAnami, S. E.
dc.date.accessioned2016-01-27T08:34:30Z
dc.date.available2016-01-27T08:34:30Z
dc.date.issued2013-07
dc.description.abstractMaize is one of the most important cereal crops in Sub-Saharan Africa and an important source of energy for humans. However, the difference in the dedifferentiation frequency of immature embryos among various genotypes indicates that callus induction and genetic transformation is dependent on the genotype. This phenomenon is an impediment in the fundamental process of improving tropical maize germplasm especially through genetic engineering. Here, five tropical maize (Zea mays L.) genotypes, CML 216, CML 144, A 04, E 04 and TL 21, were evaluated for callus induction on MS medium supplemented with the growth regulator dicamba. Embryogenic and non embryogenic callus induction was independent of genotype when young immature embryos, 12 days after pollination (DAP) were used for tissue culture in combination with dicamba. The optimal concentration of dicamba for induction of embryogenic callus in all the genotypes was 3 mg/L, which was also the concentration at which non embryogenic callus formation was lowest. The frequency of embryogenic callus induction ranged from 35% to 79% among the five genotypes and somatic embryos regenerated R0 shoots that produced normal R1 progenies. This regeneration method is expected to facilitate the development of a more efficient genotype independent Agrobacterium- mediated transformation system for tropical inbred lines.en_US
dc.identifier.citationJournal of Life Sciences,July 2013, Vol. 7, No. 7, pg. 677-689,USAen_US
dc.identifier.issn19347391
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/14091
dc.language.isoenen_US
dc.publisherDavid publishingen_US
dc.subjectTropical maizeen_US
dc.subjectGenotype independenten_US
dc.subjectDicambaen_US
dc.subjectSomatic embryogenesisen_US
dc.titleDicamba growth regulator promotes genotype independent somatic embryogenesis from immature zygotic embryos of tropical maize inbred lines.en_US
dc.typeArticleen_US
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