Molecular genotyping of selected soybean (Glycine max L.) genotypes grown in different regions of East Africa
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Date
2010-05
Authors
Njoka, Fredrick M.
Okwany, Zedekiah A.
Githui, Elijah K.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Soybean (Glycine max (L.) Merril) is one of the most popular pulses because of its protein content and
nodulating ability. However, most farmers and researchers do not have sufficient genetic information of
the genotypes that they grow. This research aimed at determining the molecular characteristics of
selected soybean genotypes grown in different regions of East Africa and make recommendations to
the farmers and researchers. Genotypes were collected from Uyole, Ukiriguru, Kawanda, Namulonge,
Njoro and Embu Agricultural Institutes as well as from farmers. Molecular analysis was done by DNA
being extracted according to phenol chloroform method. This was followed with PCR process using
custom ordered pair of primers that corresponded to the flanking ends of the targeted gene fragment
(5S ribosomal genes). Restriction fragment length polymorphism (RFLP) and gene clean were then
performed on the isolated PCR fragments. The elute was confirmed on agarose gel then sent for
automated sequencing ABI prism (Applied Biosystems) at ILRI. The resulting gene sequences were
compared with gene sequences of known Glycine species using various enzymes in computer based
simulations. The gene sequences were then subjected to gene blast using MEGA 4 and resulted with a
phylogenetic tree for the selected East African soybean. The studied cultivars were found to be closely
related to Glycine max species. Enzyme Sac11 was identified as a marker for the East African soybean
genotypes. These results may also assist plant breeders to produce hybrids with the best performing
cultivars based on their genetic diversity.
Description
Keywords
Soybean, DNA analysis, sequencing, polymorphism, markers
Citation
African Journal of Plant Science Vol. 4(5), pp. 128-134, May 2010