Show simple item record

dc.contributor.authorGimase, James M
dc.contributor.authorThagana, Wilson M
dc.contributor.authorOmondi, Chrispine O
dc.contributor.authorCheserek, Jane J
dc.contributor.authorGichuru, Elijah K
dc.date.accessioned2023-03-07T13:24:44Z
dc.date.available2023-03-07T13:24:44Z
dc.date.issued2020
dc.identifier.citationGimase, J. M., Thagana, W. M., Omondi, C. O., Cheserek, J. J., & Gichuru, E. K. (2021). Genetic relationship and the occurrence of multiple gene resistance to coffee berry disease (Colletotrichum Kahawae, Waller Bridge) within selected Coffea arabica Varieties in Kenya. African Journal of Plant Science, 15(1), 39-48.en_US
dc.identifier.issn1996-0824
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/24887
dc.descriptionArticleen_US
dc.description.abstractThe Coffee Berry Disease (CBD) epidemics destroy up to 100% of the crop on a susceptible variety. Resistance to CBD is conferred by the T-gene in Hibrido De Timor (HDT), R-gene in Rume Sudan (RS), and k-gene in K7, which were assembled in resistance varieties Ruiru 11(R11) and Batian. This study aimed to evaluate the genetic relationship between R11 and Batian with their parents' HDT, RS, SL8 and confirm the occurrence of T and R genes using DNA markers. Genome-wide single nucleotide polymorphism (SNP) markers were obtained through Diversity Arrays Technology sequencing (DArTseq). The genetic relationship was analyzed by Principal Component Analysis and hierarchical clustering. The Tgene was confirmed using Microsatellite primer, Sat 235 while the R gene was by marker sequence search within the DArTseq result files. The PC1 accounted for 42% of the total variation. Hierarchical clustering revealed less than 10% dissimilarity index apart from HDT that recorded above 20%. All the R11 and Batian genotypes carry the T gene. Eleven genotypes carry both T and R genes, therefore, with broad-based resistance to CBD. The study confirmed the narrow genetic relations within the Coffea arabica coffee varieties and further confirmed the occurrence of multiple gene resistance in R11 and Batian that will not break easily to new pathogen racesen_US
dc.language.isoenen_US
dc.publisherAfrican Journal of Plant Scienceen_US
dc.subjectCoffee berry diseaseen_US
dc.subjectdiversity arrays technology sequencingen_US
dc.subjectT geneen_US
dc.subjectR geneen_US
dc.subjectRuiru 11en_US
dc.subjectBatianen_US
dc.subjectSL 28en_US
dc.subjectcodominanten_US
dc.titleGenetic Relationship and the Occurrence of Multiple Gene Resistance to Coffee Berry Disease (Colletotrichum Kahawae, Waller & Bridge) Within Selected Coffea Arabica Varieties in Kenyaen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record