Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers
Loading...
Date
2020
Authors
Nyaboga, Evans N
Oduor, Richard O
Asande, Lydia K
Ombori, Omwoyo
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi International Journal of Agronomy
Abstract
Passion fruit (Passiflora edulis (Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the
demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a
lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports an
in vitro plant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the
regenerated plants. 'e highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented
with 2 mg·L−1 6-Benzyl amino purine (BAP) (shoot induction medium). 'e multiplication of shoots was optimum in MS
medium supplemented with 3 mg·L−1 BAP. To eliminate the requirement of an additional step of in vitro rooting, exogenous
application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of the in
vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified
polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity
of the in vitro regenerated and macropropagated plants. 'e in vitro regeneration system developed can be utilized for mass clonal
propagation for the economic commercial exploitation of this important tropical fruit.
Description
Research Article
Keywords
Citation
Asande, L. K., Ombori, O., Nyaboga, E. N., & Oduor, R. O. (2020). Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers. International Journal of Agronomy, 2020.