Antimicrobial and mechanism of antagonistic activity of Bacillus sp. A2 against pathogenic fungus and bacteria: The implication on honey's regulatory mechanism on host's microbiota
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Date
2020
Authors
Jia, Lina
Kosgey, Janet Cheruiyot
Wang, Jielin
Yang, Jianxun
Nyamao, Rose Magoma
Zhao, Yi
Teng, Xue
Gao, Lei
Wabo, MartinTherese Cheteu
Vasilyeva, Natalia V.
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
Honey is thought to act against microbes and regulates microbiota balance, and this
is mainly attributed to the enzymatic production of hydrogen peroxide, high osmolarity,
and nonperoxidase factors, for example, lysozyme and botanical sources of
nectar, while the effect of honey's probiotic is recently considered. The study of
honey as source of beneficial microbes is understudied. The purpose of this study
was to screen for the beneficial microorganisms in honey with antagonistic property
against important pathogens and the mechanism of antimicrobial activity and thus
play a beneficial role as probiotics. The results showed that one out of the fourteen
bacterial isolates had antimicrobial activity and was identified as Bacillus Sp. A2 by
16S rRNA sequence and morphology. Antimicrobial activity of the isolate against C.
albicans, E. coli, and S. aureus was confirmed by Agar well diffusion and liquid coculture
assays, and the propagation of those microbes was significantly inhibited after
treatment with the isolate Bacillus sp. A2 (p < .05) in comparison with untreated negative
control and positive control (fluconazole, chloramphenicol, L. plantarum). The
morphological changes including the distorted shape with indentations and leakages
(SEM), damaged cell membrane, and cell wall with the disintegration and attachment
of the Bacillus sp. A2 (TEM) in treated C. albicans were observed. Meanwhile, reactive
oxygen species accumulation and decreased mitochondrial membrane potential
were detected in treated C. albicans. These results revealed that the isolate Bacillus
sp. A2 from honey has significant antimicrobial activity (p < .05) against C. albicans in
comparison with untreated negative control and positive control L. plantarum, which
depends on the accumulation of reactive oxygen species, mitochondrial damage, and
the cell apoptosis. We concluded that the Bacillus sp. A2 possess the antimicrobial
property, which may contribute to regulation of host's microbiota as a beneficial microbe
or probiotic.
Description
A research article published in Food Science & Nutrition
Keywords
antagonistic bacteria, antimicrobial activity, Bacillus sp, honey, pathogenic fungus
Citation
Jia, L., Kosgey, J. C., Wang, J., Yang, J., Nyamao, R. M., Zhao, Y., ... & Fang, Y. (2020). Antimicrobial and mechanism of antagonistic activity of Bacillus sp. A2 against pathogenic fungus and bacteria: The implication on honey's regulatory mechanism on host's microbiota. Food Science & Nutrition.