Antimicrobial and mechanism of antagonistic activity of Bacillus sp. A2 against pathogenic fungus and bacteria: The implication on honey's regulatory mechanism on host's microbiota

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dc.contributor.authorJia, Lina
dc.contributor.authorKosgey, Janet Cheruiyot
dc.contributor.authorWang, Jielin
dc.contributor.authorYang, Jianxun
dc.contributor.authorNyamao, Rose Magoma
dc.contributor.authorZhao, Yi
dc.contributor.authorTeng, Xue
dc.contributor.authorGao, Lei
dc.contributor.authorWabo, MartinTherese Cheteu
dc.contributor.authorVasilyeva, Natalia V.
dc.contributor.authorFang, Yong
dc.contributor.authorZhang, Fengmin
dc.date.accessioned2020-09-16T08:01:52Z
dc.date.available2020-09-16T08:01:52Z
dc.date.issued2020
dc.descriptionA research article published in Food Science & Nutritionen_US
dc.description.abstractHoney is thought to act against microbes and regulates microbiota balance, and this is mainly attributed to the enzymatic production of hydrogen peroxide, high osmolarity, and nonperoxidase factors, for example, lysozyme and botanical sources of nectar, while the effect of honey's probiotic is recently considered. The study of honey as source of beneficial microbes is understudied. The purpose of this study was to screen for the beneficial microorganisms in honey with antagonistic property against important pathogens and the mechanism of antimicrobial activity and thus play a beneficial role as probiotics. The results showed that one out of the fourteen bacterial isolates had antimicrobial activity and was identified as Bacillus Sp. A2 by 16S rRNA sequence and morphology. Antimicrobial activity of the isolate against C. albicans, E. coli, and S. aureus was confirmed by Agar well diffusion and liquid coculture assays, and the propagation of those microbes was significantly inhibited after treatment with the isolate Bacillus sp. A2 (p < .05) in comparison with untreated negative control and positive control (fluconazole, chloramphenicol, L. plantarum). The morphological changes including the distorted shape with indentations and leakages (SEM), damaged cell membrane, and cell wall with the disintegration and attachment of the Bacillus sp. A2 (TEM) in treated C. albicans were observed. Meanwhile, reactive oxygen species accumulation and decreased mitochondrial membrane potential were detected in treated C. albicans. These results revealed that the isolate Bacillus sp. A2 from honey has significant antimicrobial activity (p < .05) against C. albicans in comparison with untreated negative control and positive control L. plantarum, which depends on the accumulation of reactive oxygen species, mitochondrial damage, and the cell apoptosis. We concluded that the Bacillus sp. A2 possess the antimicrobial property, which may contribute to regulation of host's microbiota as a beneficial microbe or probiotic.en_US
dc.identifier.citationJia, L., Kosgey, J. C., Wang, J., Yang, J., Nyamao, R. M., Zhao, Y., ... & Fang, Y. (2020). Antimicrobial and mechanism of antagonistic activity of Bacillus sp. A2 against pathogenic fungus and bacteria: The implication on honey's regulatory mechanism on host's microbiota. Food Science & Nutrition.en_US
dc.identifier.issn2048-7177
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/pdf/10.1002/fsn3.1770
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/20362
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectantagonistic bacteriaen_US
dc.subjectantimicrobial activityen_US
dc.subjectBacillus spen_US
dc.subjecthoneyen_US
dc.subjectpathogenic fungusen_US
dc.titleAntimicrobial and mechanism of antagonistic activity of Bacillus sp. A2 against pathogenic fungus and bacteria: The implication on honey's regulatory mechanism on host's microbiotaen_US
dc.typeArticleen_US
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