Genetic Diversity Studies on Selected Rice (Oryza sativa L.) Genotypes based on Gel Consistency and Alkali Digestion
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Date
2016
Authors
Chemutai, L.R.
Musyoki, M.A.
Kioko, W.F.
Mwenda, N.S.
Muriira, K.G.
Ngugi, M.P.
Journal Title
Journal ISSN
Volume Title
Publisher
OMICS International
Abstract
Knowledge of rice genetic diversity is necessary to ascertain the germplasm conservation and the development of
improved rice genotypes with good quality traits through various breeding programs. The aim of this study was to
determine the genetic diversity based on gel consistency and alkali digestion among selected Kenyan and
Tanzanian genotypes using SSR markers. PowerMarker version 3.25, GenAlEx version 6.41 and DARwin 6.0.12
statistical software were used to carry out data analysis. The number of alleles per locus ranged from 2 to 4 with an
average of 2.75 across the 8 markers used. Polymorphic information content (PIC) ranged from 0.5224 (RM577) to
0.1411 (RM85) with an average of 0.3673 observed across all the markers. Gene diversity ranged from 0.5764
(RM577) to 0.1528 (RM85) with an average of 0.4181 with one rare allele was detected at RM577 loci. Pairwise
genetic dissimilarity matrix ranged from ranged from 0.9333 to 0.1818 with the least genetic distance being observed
between IR 54 and BS 370 while the highest, 0.9333 being between Saro 5 and IR 2793. The unweighted neighbour
joining tree clustered the rice genotypes into three major clusters and subsequent sub clusters hence effectively
differentiating the Kenyan and Tanzanian genotypes based on gel consistency and alkali digestion. This clustering
was complemented with the findings in the principal coordinate analysis. These results show that determination of
genetic diversity using SSR markers cam be successfully achieved. In this study RM577 was the best and most
informative SSR marker given it showed the highest PIC, gene diversity and allele per locus. In addition, it’s the only
marker that showed a rare allele at 400 bp.
Description
Research Article
Keywords
Rice, Genetic diversity, Gel consistency, Alkali digestion
Citation
Chemutai LR, Musyoki MA, Kioko WF, Mwenda NS, Muriira KG, et al. (2016) Genetic Diversity Studies on Selected Rice (Oryza sativa L.) Genotypes based on Gel Consistency and Alkali Digestion. J Rice Res 4: 172. doi:10.4172/2375-4338.1000172