RP-Department of Environmental Health
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Browsing RP-Department of Environmental Health by Subject "Aflatoxin"
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Item Aflatoxin Bioaccumulation in Broiler Chicken Meat and Organs from Selected Farms in Nairobi City County, Kenya(International Organization Of Scientific Research (IOSR), 2023) Chepkosgei, Kirinyet Ruth; Njogu, Warutere Peterson; Nguhiu, Purity; Ojola, Patroba; Kirinyet, Joel; Koskei, Peter; Ndiritu, Alex KaruiruBackground: Food safety is a worldwide concern not only to policymakers but also to the general population. Food is regarded as safe when there is assurance that no damage will accrue from its consumption. Aflatoxins pose a major risk to the health of both humans and animals. Studies on residue levels in broiler chicken are limited in Kenya hence grounded on this knowledge, this study sought to determine aflatoxin residue levels in broiler meat and organs in Nairobi City County. Materials and Methods: The study employed a longitudinal study design for a period of six weeks. A total of 42 broilers were sampled. Samples were analyzed using the LC-MS/MS technique. Data was analyzed using STATA version 12. Tukey Kramer post hoc test was used for comparison of means and statistical significance was determined at 5%. Ethical approval was sought from relevant authorities before commencement of the study and consent was sought from the participants before taking part in the study. Results: Results from the study show that there was a significant association (p<0.05) in AFB1 and Total Aflatoxin levels in the gizzard, liver and muscle per week. AFB1 levels in the gizzard were below the WHO/FAO limit of 5 ppb however they were above the EU limit of 2ppb in week 5 and 6. In the liver AFB1 levels were above the EU limit in week 4, 5 and 6 and above the WHO/FAO limit in week 6.In the muscle AFB1 levels were all below the WHO/FAO and EU limit. Total Aflatoxin levels in the liver were above the EU limit in week 4, 5 and 6 and above the WHO/FAO limit in week 6. In the muscle Total Aflatoxin levels were all below the WHO/FAO and EU limit. Conclusion:The results of the study suggest that there were appreciable levels of aflatoxin in the liver and gizzard hence there is need for continuous surveillance and monitoring of aflatoxin levels in feed by regulatory bodies, county and national government to prevent carry over in meat.Item Carry Over Effect of Aflatoxin from Broiler Feed to Broiler Meat and Organs in Chicken raised in Nairobi City County, Kenya(IOSR, 2023) Chepkosgei, Kirinyet Ruth; Njogu, Warutere Peterson; Purity, NguhiuBackground: Aflatoxin is a threat and a food safety concern particularly in developing countries due to the climatic conditions that favor the growth of the aflatoxin fungi. Consequently, this is a major risk to feed ingredients used in the manufacture of animal feed and subsequently a great risk to human consumers due to the detrimental effects of these toxins. Since there are no documented studies on carry over effect of aflatoxin in broilers in Kenya, a study to establish the carry over effect of aflatoxin in broiler chicken was carried out in Nairobi City County. Materials and Methods: The study employed a longitudinal study design where by broiler chicken were followed for a period of six weeks. The broilers were sampled from six farms in six sub counties within Nairobi City County hence, a total of 42 birds were sampled.The samples obtained were meat (muscle, liver and gizzard), feed and water and were analyzed using the LC-MS/MS technique to determine the Aflatoxin levels.The carry over effect in this study was determined statistically. STATA version 12 was used to analyze the data. Tukey Kramer post hoc test was used for comparison of means and statistical significance was determined at 5%. Results:There was a statistical significant difference (p<0.05) in the carry over ratio of aflatoxin per week. The highest carry over ratio of >10% was observed in the liver, followed by the gizzard and the least was in the muscle.The highest transfer ratio was observed in week 5 and 6 in the liver and in week 6 in the muscle. The carry over ratio in the muscle was below 1%. Conclusion:The study concludes that it is less safe to consume the liver and gizzard as the transfer is high however it is safer to consume the muscle as the transfer is low. There is need to constantly monitor aflatoxin levels in feed and feed ingredients hence prevent carry over into animal tissues consequently reducing risk to humans.Item Determination of Knowledge Based on Aflatoxin Poisoning among Broiler Farmers in Nairobi City County, Kenya(Microbiology Research Journal International, 2023) Chepkosgei, Ruth; Njogu, Warutere Peterson; Nguhiu, Purity; Ojola, Patroba; Kirinyet, Joel; Koskei, PeterBackground: There is a scarcity of information concerning knowledge of aflatoxin contamination of feeds among farmers even in aflatoxin-prone regions in Kenya. Thus, knowledge of aflatoxins in feeds among poultry farmers is of paramount importance in designing plans to minimize risks of aflatoxin exposure. Therefore, this study sought to assess the Determinants of Knowledge on Aflatoxin Among Broiler farmers in Nairobi City County, Kenya. Methodology: The study utilized an analytical cross-sectional study design. A total of 240 farmers were sampled from a population of 600 farmers within Nairobi City County. A structured questionnaire was administered to farmers within Nairobi City County. SPSS version 26 was used to analyze the data descriptively. Results were presented in tables and figures. Ethical approval was sought from relevant authorities and parties before the commencement of the study. Results: Results from the study show that the majority of the farmers (58.2%) had knowledge of aflatoxin. There was a significant association (p<0.05) between the socio-demographic characteristics of farmers and knowledge of aflatoxin. Conclusion: The study concludes that the farmers had adequate knowledge of aflatoxin occurrence in feeds and methods to reduce the contamination. There is a need for continuous sensitization of farmers on aflatoxin, particularly on feed management practices by the Ministry of Agriculture and Ministry of Health Division of Public Health in Kenya