Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)

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dc.contributor.authorKamau, L. M.
dc.contributor.authorSkilton, R. A.
dc.contributor.authorOdongo, D.O.
dc.contributor.authorMwaura, S.
dc.contributor.authorGithaka, N.
dc.contributor.authorKanduma, E.
dc.contributor.authorObura, M.
dc.contributor.authorOrago, A. S.
dc.contributor.authorKabiru, Ephantus W.
dc.contributor.authorMusoke, A.
dc.contributor.authorBishop, R. P.
dc.date.accessioned2013-10-29T13:32:35Z
dc.date.available2013-10-29T13:32:35Z
dc.date.issued2011
dc.descriptiondoi: 10.1111/j.1365-2583.2010.01043.xen_US
dc.description.abstractThe transcriptional control of gene expression is not well documented in the Arthropoda. We describe tran-scriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhi-picephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of Rhipicephalus (Boophilus) microplus. The R. appendiculatus Ra86 proteins contain 654 and 693 amino acids, with only 80% amino acid sequence identity. Reverse-transcription PCR of gut cDNA showed transcription of only one genotype in individual female ticks. PCR amplification of 3 untranslated sequences from genomic DNA indicated that both variants could be encoded within a single genome. When both variants were present, one of the two Ra86 genotypes was transcriptionally dominant.en_US
dc.identifier.citationInsect Molecular Biology (2011) 20 (1), 105–114en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/7547
dc.language.isoenen_US
dc.publisherInsect Molecular Biologyen_US
dc.subjectRhipicephalus appendiculatusen_US
dc.subjectTicken_US
dc.subjectVaccineen_US
dc.subjectGut antigenen_US
dc.subjectRa86en_US
dc.subjectDifferential transcriptionen_US
dc.titleDifferential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)en_US
dc.typeArticleen_US
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