Optimisation of parameters for agrobacterium-mediated  transformation of sweet potato

dc.contributor.author	Machuka, Jesse
dc.contributor.author	Njagi, W.
dc.contributor.author	Gichuki, S. T.
dc.contributor.author	Macharia, C.
dc.contributor.author	Muluvi, G. M.
dc.date.accessioned	2014-07-15T08:15:40Z
dc.date.available	2014-07-15T08:15:40Z
dc.date.issued	2010
dc.description.abstract	An Agrobacterium-mediated transformation and somatic regeneration protocol was adapted for a Kenyan sweet potato variety, KSP36. A model cultivar, CTP560 was used as a control. For selection of transformed explants paramomycin was found to be effective at 25mg/L while kanamycin was effective at 20mg/L. The lower concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations proved better for regeneration as opposed to the higher 2,4-D concentrations. Zeatin/ IAA (indole acetic acid) was more effective at embryo production as opposed to kinetin/ 2,4-D medium in both cultivars. Out of the 18 KSP36 plants tested by PCR, 11 tested positive for the coat protein gene while 9 out of the 19 CPT560 plants tested positive. This protocol can be recommended for other sweet potato varieties.	en_US
dc.identifier.citation	Proceedings of the 10th KARI biennial scientific conference	en_US
dc.identifier.uri	http://www.kari.or.ke/fileadmin/publications/10thproceedings/Poster/Optmisation_Paramts_Agrob.pdf
dc.identifier.uri	http://ir-library.ku.ac.ke/handle/123456789/10403
dc.language.iso	en	en_US
dc.title	Optimisation of parameters for agrobacterium-mediated transformation of sweet potato	en_US
dc.type	Article	en_US

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