Activation of peroxisome proliferator– activated receptor gamma induces anti-inflammatory properties in the chicken free avian respiratory macrophages
Loading...
Date
2015
Authors
Mutua, Mbuvi P.
Steinaa, Lucilla
Muya, Shadrack M.
Gicheru, Muita M.
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Background: Activation of peroxisome proliferator activated receptor gamma (PPAR γ) in the alveolar macrophages
(AM) by selective synthetic PPAR γ ligands, improves the ability of the cells to resolve inflammation. In birds,
respiratory macrophages are known as free avian respiratory macrophages (FARM) and show distinct functional
differences from AM. The effects of treating FARM with PPAR γ ligands are unclear.
Methods: FARM were harvested by lavage of chicken respiratory tract and their morphology assessed at
microscopic level. The effects of PPAR γ agonists on the FARM in vitro viability, phagocytic capacity and
proinflammatory cytokine (TNF-α) production were assessed.
Results: FARM had eccentric nucleus and plasma membrane ruffled with filopodial extensions. Ultrastructurally,
numerous vesicular bodies presumed to be lysosomes were present. FARM treated with troglitazone, a selective
PPAR γ agonist, had similar in vitro viability with untreated FARM. However, treated FARM co-cultured with
polystyrene particles, internalized more particles with a mean volume density of 41 % compared to that of
untreated FARM of 21 %. Further, treated FARM significantly decreased LPS-induced TNF-α production in a dose
dependent manner.
Conclusion: Results from this study show that PPAR γ synthetic ligands enhance phagocytic ability of FARM.
Further the ligands attenuate production of proinflammatory cytokines in the FARM, suggesting potential
therapeutic application of PPAR γ ligands in the management of respiratory inflammatory disorders in the poultry
industry.
Description
DOI: 10.1186/s40781-015-0073-1
Keywords
Avian, Free avian respiratory macrophages, Peroxisome proliferator-activated receptor, Troglitazone
Citation
Journal of Animal Science and Technology (2015) 57:40