Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma

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dc.contributor.authorMwangi, Catherine
dc.contributor.authorNjoroge, Stephen
dc.contributor.authorTshibangu-Kabamba, Evariste
dc.contributor.authorMoloo, Zahir
dc.contributor.authorRajula, Allan
dc.contributor.authorDevani, Smita
dc.contributor.authorMatsumoto, Takashi
dc.contributor.authorNyerere, Kimang’a
dc.contributor.authorKariuki, Samuel
dc.contributor.authorRevathi, Gunturu
dc.contributor.authorYamaoka, Yoshio
dc.date.accessioned2020-09-21T08:44:58Z
dc.date.available2020-09-21T08:44:58Z
dc.date.issued2020
dc.descriptionThis research was funded by the Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (26640114, 15H02657, 16H05191, 16H06279, 18KK0266, and 19H03473 to Yoshio Yamaoka), the Special Coordination Funds for Promoting Science and Technology from the MEXT of Japan to Yoshio Yamaoka, and National Institutes of Health grants DK62813 to Yoshio Yamaoka. This work was also supported by the Japan Society for the Promotion of Science (JSPS) Institutional Program for Core-to-Core Program; B. Africa-Asia Science Platform to Yoshio Yamaoka, and the Oita University President Research Project 2019 to Takashi Matsumoto, Evariste Tshibangu-Kabamba is a doctoral student supported by the MEXT Scholarship Program founded by the Japanese Government.en_US
dc.description.abstractHelicobacter pylori(H.pylori) infection is etiologically associated with severe diseases including gastric cancer; but its pathogenicity is deeply shaped by the exceptional genomic diversification and geographic variation of the species. The clinical relevance of strains colonizing Africa is still debated. This study aimed to explore genomic features and virulence potentials of H. pylori KE21, a typical African strain isolated from a native Kenyan patient diagnosed with a gastric cancer. A high-quality circular genome assembly of 1,648,327 bp (1590 genes) obtained as a hybrid of Illumina Miseq short reads and Oxford Nanopore MinION long reads, clustered within hpAfrica1 population. This genome revealed a virulome and a mobilome encoding more than hundred features potentiating a successful colonization, persistent infection, and enhanced disease pathogenesis. Furthermore, through an experimental infection of gastric epithelial cell lines, strain KE21 showed the ability to promote interleukin-8 production and to induce cellular alterations resulting from the injection of a functional CagA oncogene protein into the cells. This study shows that strain KE21 is potentially virulent and can trigger oncogenic pathways in gastric epithelial cells. Expended genomic and clinical explorations are required to evaluate the epidemiological importance of H. pylori infection and its putative complications in the study population.en_US
dc.identifier.citationMwangi, C., Njoroge, S., Tshibangu-Kabamba, E., Moloo, Z., Rajula, A., Devani, S., ... & Yamaoka, Y. (2020). Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma. Toxins, 12(9), 556.en_US
dc.identifier.issn2072-6651
dc.identifier.urihttps://doi.org/10.3390/toxins12090556
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/20402
dc.language.isoenen_US
dc.publisherMPDIen_US
dc.subjectHelicobacter pylorien_US
dc.subjectKenyaen_US
dc.subjectwhole genome sequencingen_US
dc.subjectvirulenceen_US
dc.subjectvirulomeen_US
dc.subjectmobilomeen_US
dc.subjectIllumina Miseqen_US
dc.subjectOxford Nanopore MinIONen_US
dc.titleWhole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinomaen_US
dc.typeArticleen_US
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