Plant regeneration via somatic embryogenesis in mature embryo-derived callus culture of Sinocalamus latiflora (Munro) McClure
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Date
1987
Authors
Yeh, Meei-Ling
Chang, Wei-Chin
Journal Title
Journal ISSN
Volume Title
Publisher
Science Direct
Abstract
Somatic embryogenesis and subsequent formation of plantlets was achieved from callus cultures derived from mature zygotic embryos of Sinocalamus latiflora (Munro) McClure (Bamboo). Embryogenic callus was initiated on Murashige and Skoog's medium (MS) supplemented with 6 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 3 mg/l kinetin, 250 mg/l polyvinylpyrrolidon and 5% sucrose. Prolonged culture of the embryogenic callus on the same medium resulted in embryoid formation. The embryoids developed further to yield whole plantlets when transferred to a medium containing lower concentrations of 2,4-D (3 mg/l) and kinetin (2 mg/l).
Description
Keywords
Sinocalamus latiflora (Munro) McClure, somatic embryogenesis, embryoid, plant regeneration, bamboo
Citation
Plant Science Volume 51, Issue 1, 1987, Pages 93–96