Interaction of cassava mosaic disease and cassava brown streak disease in nicotiana benthamiana

Simple item page
dc.contributor.authorNgeranwa, J.J.N.
dc.contributor.authorIrungu, J.
dc.contributor.authorMiano
dc.contributor.authorMbogo, E.
dc.contributor.authorMonjero, K.
dc.contributor.authorGichuki, S. T.
dc.date.accessioned2014-07-14T13:08:40Z
dc.date.available2014-07-14T13:08:40Z
dc.date.issued2010
dc.description.abstractThe interaction of Cassava mosaic geminivirus (CMG)and Cassava brown streak virus (CBSV) were tested in Nicotiana benthamiana. The inoculated virus and/or virus species were CMGs species (ACMV and EACMV-UgV), CBSV and dual CMGs and CBSV. The inoculum source was N. benthamianaplants pre-infected with viruses from diseased cassava cultivars collected from the western region of Kenya. Leaf samples from virus-infected N. benthamiana plants were ground in inoculation buffer [0.1M K-phosphate buffer, pH 7.0, containing 0.01% (w/v) βME and Na2SO3]. The leaf homogenates were rubbed on 2-3 carborundum-dusted leaves of Nicotiana benthamiana plants at 3-5 leaf stage. Four treatments, CMGs (UgV+ACMV); CBSV; combination of CMGs+CBSV and non inoculated control plants were applied with 10 plants per each treatment inoculated in two trials. The plants were grown in a screen house and data recorded on severity of symptoms on leaves, days to symptom appearance and the percentage of infected plants. Highly significant p<0.05 difference were observed on the severity of the disease but differences in days to first appearance of symptoms and the number of infected plants were not significant p<0.05. The most severe symptoms of the disease and number of infected plants were observed in plants infected with both CMGs and CBSV (4.7 and 8) respectively. The earliest symptoms appeared in plants infected with CBSV (5 dpi) and dual infection took the longest period before symptoms were observed (9dpi). None of the plants in the control exhibited leaf symptoms. The dually infected plants exhibited more severe symptoms compared to single infections indicating synergistic interaction when the two viruses occur in combinationen_US
dc.identifier.citationProceedings of The 12TH KARI Biennial Scientific Conference “Transforming Agriculture for improved livelihoods through Agricultural Product Value Chains”en_US
dc.identifier.urihttp://www.kari.org/conference/conference12/docs/INTERACTION%20OF%20CASSAVA%20MOSAIC%20DISEASE%20AND%20CASSAVA%20BROWN%20STREAK.pdf
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/10397
dc.language.isoenen_US
dc.subjectCassava mosaic diseaseen_US
dc.subjectCassava mosaic geminivirusesen_US
dc.subjectCassava brown streak virusen_US
dc.subjectinoculationen_US
dc.subjecttransmissionen_US
dc.subjectNicotiana benthamianaen_US
dc.subjectdays post-inoculationen_US
dc.titleInteraction of cassava mosaic disease and cassava brown streak disease in nicotiana benthamianaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Interaction of cassava mosaic disease...pdf
Size:
50.13 KB
Format:
Adobe Portable Document Format
Description:
abstract
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
CW-Department of Biochemistry and Biotechnology