Ethnobotanical Identification and Anti-microbial Evaluation of Some Anti- Diarrhoreal Plants Used by the Samburu Community, K enya
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Date
2012
Authors
Okemo, P. O.
Omori, E. O.
Ogol, C.K.P.O.
Mbugua, P. K.
Journal Title
Journal ISSN
Volume Title
Publisher
Malaysian Society for Microbiology
Abstract
Aims: The anti bacterial effect of some selected Samburu medicinal plants were evaluated on bacterial strains like Staphylococcus aureus-ATCC 20591, Bacillus subtillis-local isolate, Salmonella typhi-ATCC 2202, Escherichia coli-STD25922 and Pseudomonas aeruginosa ATCC 25852.
Methodology and Results: Methanol was used as an extraction solvent for the medicinal plants after they had been dried and ground using the laboratory grinding miller. The in vitroanti bacterial activity was performed by agar discdiffusion method. The significant anti microbial activity of active extracts was compared with the standard antibiotics, cefrodoxima and amoxicillin. These results were significant at p< 0.01. The MICs were also determined by the microtitre plate method. The MICs of the most active plants ranged from 18.75 mg/mL to 37.5 mg/mL. The MBCs ranged between 18.75 mg/mL to 37.5 mg/mL. The most active plant that had substantial activity in at least allthe test cultures was found to be Lannea triphylla (A.Rich) Engl. A number of phytochemicals were also found to be present with tannins being the most abundant followed by flavonoids and saponins.
Conclusion, significance and impact of study: The findings show that most of the medicinal plantsused by the Samburu community have some significant activity onthe bacterial isolates known to cause diarrhoea.
Description
Keywords
diarrhoea, medicinal plants, phytochemicals, antimicrobial activity, ethnobotany
Citation
Malaysian Journal of Microbiology, Vol 8(2) 2012, pp. 68 74