Serotype and Genetic Diversity of Human Rhinovirus Strains that Circulated in Kenya in 2008

Simple item page
dc.contributor.authorMilanoi, Sylvia
dc.contributor.authorOngus, Juliette R.
dc.contributor.authorGachara, George
dc.contributor.authorColdren, Rodney
dc.contributor.authorBulimo, Wallace
dc.date.accessioned2016-02-02T12:19:02Z
dc.date.available2016-02-02T12:19:02Z
dc.date.issued2016
dc.descriptionDOI: 10.1111/irv.12373en_US
dc.description.abstractBackground: Human Rhinoviruses (HRVs) are a well-established cause of the common cold and recent studies indicated that they may be associated with severe acute respiratory illnesses (SARIs) like pneumonia, asthma and bronchiolitis. Despite global studies on the genetic diversity of the virus, the serotype diversity of these viruses across diverse geographical regions in Kenya has not been characterized. Objectives: This study sought to characterize the serotype diversity of HRV strains that circulated in Kenya in 2008. Methods: A total of 517 archived nasopharyngeal samples collected in a previous respiratory virus surveillance program across Kenya in 2008 were selected. Participants enrolled were outpatients who presented with influenza-like (ILI) symptoms. Real time RT-PCR was employed for preliminary HRV detection. HRV positive samples were amplified using RT-PCR and thereafter the nucleotide sequences of the amplicons were determined followed by phylogenetic analysis. Results: Twenty five percent of the samples tested positive for HRV. Phylogenetic analysis revealed that the Kenyan HRVs clustered into three main species comprising HRV A (54%), HRV-B (12%) and HRV C (35%). Overall, 20 different serotypes were identified. Intra-strain sequence homology among the Kenyan strains ranged from 58% to 100% at the nucleotide level and 55% to 100% at the amino acid level. Accepted Article This article is protected by copyright. All rights reserved. Conclusion: These results show that a wide range of HRV serotypes with different levels of nucleotide variation were present in Kenya. Furthermore, our data show that HRVs contributed substantially to influenza-like illness in Kenya in 2008.en_US
dc.identifier.citationInfluenza Other Respir Viruses; 2016en_US
dc.identifier.issn1750-2640
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/26822469
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/14105
dc.language.isoenen_US
dc.publisherWiley Open Accessen_US
dc.subjectDiversityen_US
dc.subjectGeneticen_US
dc.subjectKenyaen_US
dc.subjectRhinovirusen_US
dc.subjectSerotypeen_US
dc.titleSerotype and Genetic Diversity of Human Rhinovirus Strains that Circulated in Kenya in 2008en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Serotype and genetic diversity of human rhinovirus........pdf
Size:
636.26 KB
Format:
Adobe Portable Document Format
Description:
Full Text Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Deparment of Medical Laboratory Sciences