Isolation, characterization and primary screening of soil actinomycetes from Kenyatta University arboretum grounds for antibacterial activities

Simple item page
dc.contributor.authorKhasabuli, O. Y.
dc.contributor.authorKibera, A. N.
dc.date.accessioned2014-05-20T06:03:25Z
dc.date.available2014-05-20T06:03:25Z
dc.date.issued2014
dc.description.abstractObjective: Antimicrobial resistance has increased drastically in recent years in the developing countries, and it has leading public health concern. With almost all organisms becoming multidrug resistant to the commonly used antibiotics, there is a need to search for more drugs that are novel in order to address this challenge. Therefore, this study was aimed at isolating antimicrobial producing actinomyscetes and to evaluate their secondary metabolites against selected multidrug resistant bacteria isolates. Methodology: Half kg soil samples were collected 15cm below the ground. Starch Casein Agar (SCA) was used to isolate actinomycetes. Fifteen different actinomycetes identified as Is.1-Is.15 were isolated. They were differentiated based on the difference in appearance of the colony morphology and mycelial structure. Their metabolites were tested for antibiotic activities through the primary screening using the disk diffusion methods. Test bacteria were; Bacillus subtilis, E.coli, Klebsiella Pneumoniae, Staphylococcus aureus, Shigella sonei and Salmonella typhi. All the secondary metabolites from the isolated actinomycetes proved to posses the ability to inhibit bacterial growth by formation of different sized halo zones. Conclusion and application: Secondary metabolites produced by actinomycetes have the ability to inhibit growth of pathogenic bacteria. The ability of these metabolites to inhibit growth of test pathogenic bacteria has been attributed to the establishment of the fact that novel antibiotics can be manufactured from metabolites produced by actinomycetes. Large scale manufacture can be done through purification of these metabolites to ensure large scale production of novel drugs. This can serve help clear pathogenic bacteria that are ever developing resistance on the available antibiotics.en_US
dc.identifier.citationJournal of Applied Biosciences 74:6072– 6079en_US
dc.identifier.issn1997–5902
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/9526
dc.language.isoenen_US
dc.publisherF.a.C.T Ltden_US
dc.subjectActinomycetesen_US
dc.subjectmetabolitesen_US
dc.subjectsoilen_US
dc.subjectantimicrobial activityen_US
dc.titleIsolation, characterization and primary screening of soil actinomycetes from Kenyatta University arboretum grounds for antibacterial activitiesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Isolation, characterization and primary screening of....pdf
Size:
416.57 KB
Format:
Adobe Portable Document Format
Description:
Full text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Chemistry