Antimicrobial evaluation of crude methanolic leaf extracts from selected medicinal plants against escherichia coli
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Date
2016-01
Authors
Opinde, Hibert Rachuonyo
Gatheri, G. W.
Nyamache, A. K.
Journal Title
Journal ISSN
Volume Title
Publisher
OMICS International
Abstract
This study was aimed at determining the antibacterial effects of the selected plant leaf extracts of Tagetes minuta,
Aloe secundiflora, Vernonia lasiopus and Bulbine frutescens against clinical isolate of Escherichia coli. The plants
materials were obtained from Kenyatta University arboretum and identified by University taxonomist and voucher
specimen deposited in the University. Methanol was used as the solvent for the extraction process and the
antimicrobial activity test carried out using the disc diffusion method. All the plants extracts analysed had
antimicrobial activity against Escherichia coli with Tagetes minuta extract being the most active at low concentrations
(8.7 mg/ml). The standard antibiotic used for positive control was ciprofloxacin (5 μg/ml) while distilled water and
dimethyl sulphoxide were used as negative control. Screening of phytochemical showed the presence of four
phytochemicals; saponins, tannins, alkaloids and flavonoids.
Description
http://dx.doi.org/10.4172/2155-9597.1000272
Keywords
Disc diffusion method, Phytochemicals, Escherichia coli, Ciprofloxacin, Dimethyl sulphoxide
Citation
Journal of Bacteriology & Parasitology, J Bacteriol Parasitol 2016: http://dx.doi.org/10.4172/2155-9597.1000272