Prevalence and Genetic Diversity of Hepatitis B and C Viruses among Human Immunodeficiency Virus Infected Individuals in Siaya County, Kenya

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dc.contributor.authorOnyango, Omondi Kevin
dc.date.accessioned2021-02-16T09:50:34Z
dc.date.available2021-02-16T09:50:34Z
dc.date.issued2020-09
dc.descriptionA Thesis Submitted in Fulfillment of the requirements for the award of the degree of Master of Science (Microbiology) in the School of Pure and Applied Sciences of Kenyatta University September, 2020en_US
dc.description.abstractViral hepatitis B and C co-infections among Human immunodeficiency virus infected patients is significantly becoming a worrying public health problem worldwide. The three infectious viruses’ shares common routes of transmission including, blood transfusion, sexual intercourse, and injecting drug users among others, which could be the reason for the co-infections observed in the previous studies in sub-Sahara Africa. In Kenya, the documented data from the studies conducted indicate that Nyanza recorded the highest HIV prevalence at 15.1% with Siaya County at 17.8% of the total national population. The invention of Highly Active Antiretroviral Therapy was thought to reduce morbidity and mortality rate of HIV patients, however, HIV patients continue to suffer from liver related illness due to the co-infections with hepatitis B and C viruses, in addition to the emergence of drug resistant strains. Despite this there is scarcity of information on hepatitis B and C co-infections, circulating virus genotypes and drug resistant strains in Kenya. This study evaluated the co-infections of viral hepatitis B/C and genetic diversity and drug resistance of HBV among HIV infected individuals in Siaya County. Approval to conduct this study was sought from Kenyatta University Research and Ethical Review Committee and SCRH Institutional review Committee. This was a hospital based cross-sectional study in which a total of 225 blood samples were aseptically collected from consenting participants. The blood samples were separated and plasma used for serological assays. Serological detection of HBsAg and anti-HCV IgM was performed using On Site Rapid Test Kits as prescribed by the manufacture (CTK Biotech, Inc, San Diego, USA).Viral DNA was extracted from positive HBsAg plasma samples using QiampTMDNA Mini kit as per the manufactures’ instructions. HBV-pol gene was amplified by nested PCR using specific primers and the amplicons directly sequenced by automated ABI 377 DNA sequencer (Applied Biosystem, Foster City, USA) using BigDye Terminator Kit (Applied Biosystem®). Generated sequences were phylogenetically analyzed together with references sequences using Molecular Evolutionary Genetics Analysis (MEGA X version 10.0.4) software. Of (225) individuals who participated in this study, 157(69.8%) were female and 68(30.2%) were males. Their ages were ranged between 3 and 76 years with mean of 38.26 years. Majority of the participants were married (146/225) with most of them having secondary education level (116/225). Gender, age and level of education were not significantly associated with HBV infection. However, place of residence was associated with HBV infection. In addition, only gender and marital status were significantly associated with HCV infections. Overall prevalence for HBV/HIV was 6.2% (14/225); HCV/HIV was 4.0% (9/225) while that of HIV mono-infection was 89.8% (202/225). Nevertheless, none of the study participants was infected with all the three viruses. HBV drug resistance mutation rt169F was detected in one participant. However, the rest of the 10 Individuals were infected with HBV drug susceptible strains. Of the 11 samples that were successfully sequenced, the phylogenetic analysis revealed the sequences belonged to HBV genotype A1. The study findings reveal that the levels of HBV/HIV and HIV/HCV co-infections could be higher than reported here with circulating strains remaining susceptible to treatment. There is therefore a need for continuous surveillance of HBV, HCV infections and monitoring circulating trends of these viral genotypes and drug resistance in this region in order to guide vaccine design and optimize treatment.en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/21471
dc.language.isoenen_US
dc.publisherKenyatta Universityen_US
dc.subjectPrevalenceen_US
dc.subjectGenetic Diversityen_US
dc.subjectHepatitis B and Cen_US
dc.subjectVirusesen_US
dc.subjectHuman Immunodeficiency Virus Infecteden_US
dc.subjectIndividualsen_US
dc.subjectSiaya Countyen_US
dc.subjectKenyaen_US
dc.titlePrevalence and Genetic Diversity of Hepatitis B and C Viruses among Human Immunodeficiency Virus Infected Individuals in Siaya County, Kenyaen_US
dc.typeThesisen_US
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