Prevalence and Risk Factors Associated with Geohelminths and Intestinal Protozoa Infections among Pupils Aged 7–12 Years in Nyeri County, Kenya

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Date
2025-10
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Kenyatta University
Abstract
Helminths and protozoa are the two categories into which intestinal parasites fall. Schoolage children are highly susceptible to intestinal protozoa and soil-transmitted helminth (STH) infections. Geo-helminth and intestinal protozoa infections remain a significant public health burden in many parts of Kenya, especially among school-going children in rural and peri-urban regions. Despite the implementation of Mass Drug Administration (MDA) programs, the prevalence of these infections among school-aged children continues to rise. The study aimed to determine the prevalence and associated risk factors of STH and intestinal protozoa infections among school-going children in selected primary schools in Konyu area. The Specific Objectives were: To determine the prevalence of geohelminths and intestinal protozoa infection among school children; to identify the risk factors associated with geohelminths and intestinal protozoa infection among pupils, and To identify the environment related risk factors associated with geohelminths and intestinal protozoa infection in Nyeri County. The Epidemiological Triangle Model (ETM) guided this study. A cross-sectional study design was employed involving 164 pupils from Kihuro, Gathuini, and Gikumbo primary schools. The study was conducted in 3 primary schools which were purposively selected and the study population comprised pupils in Grades 1 to 6, aged between 7 and 12 years. This study employed a cluster random sampling technique to select participants from public primary. Using the Cochran formula, a total number of 178 study subjects was calculated, though only 164 pupils provided complete date. Pre-testing of the research instruments was conducted in a school setting, to ensure consistency with the actual study environment. The reliability of the study tools and laboratory procedures, quality control measures were implemented and a coefficient of 0.84 was obtained. The data collection process began with a community sensitization meeting supported by local leaders, followed by a focus group discussion involving key stakeholders. Pupils and their guardians completed structured questionnaires on hygiene and environmental risk factors before being given labeled containers for stool sample collection. Samples were collected and analysed using the Kato-Katz technique for helminths and the Formal Ether Concentration technique for protozoa. Laboratory SOPs were rigorously followed, with quality checks to ensure accuracy and confidentiality. Data were cleaned, coded, and analysed using SPSS version 28.0. Descriptive statistics were used to summarize participant characteristics, while Chisquare tests evaluated associations between risk factors and infection prevalence. The prevalence of intestinal protozoa was 21.4%, 33%, and 20.4% in Kihuro, Gathuini, and Gikumbo, respectively, while STH prevalence was 10.7%, 13%, and 13%. There was a significant association between pupil and environmental risk factors and the prevalence of infections (p < 0.05). Risk factors related to hygiene practices, sanitation, and environmental exposure were significantly associated with infection rates, underscoring the need for integrated interventions beyond routine deworming. The study concluded that infection prevalence was high and significantly linked to personal and environmental risk behaviours, leading to targeted recommendations for health education, hygiene infrastructure, regular deworming, and improved WASH practices in schools.
Description
A Research Thesis Submitted in Partial Fulfilment of the Requirement for the Award of the Degree of Master of Public Health (Epidemiology and Disease Control) in the School of Health Sciences of Kenyatta University, October, 2025 Supervisor: 1.Harun Kimani 2.Martin Mutuku
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