An isocratic reversed-phase HPLC separation of the stereoisomers of the provitamin A carotenoids (α- and β-carotene) in dark green vegetables

dc.contributor.author	Nyambaka, H. N.
dc.contributor.author	Ryley, J.
dc.date.accessioned	2014-06-18T08:38:56Z
dc.date.available	2014-06-18T08:38:56Z
dc.date.issued	1996
dc.description	DOI: 10.1016/0308-8146(95)00093-3	en_US
dc.description.abstract	An isocratic reversed phase HPLC assay was developed for monitoring the isomers of α- and β-carotene in dark green leafy vegetables using a mixture of isomers obtained from the light catalysed oxidation of all-trans α- and β-carotene in the presence of iodine. The method was applied to Italian spinach, spring cabbage and cowpea leaves. The isomers were identified by the elution order from thin layer chromatography, the absorption spectral data and comparison with published results. The three main isomers of β-carotene found to be dominant in the samples analysed were all-trans, 9-cis and 13-cis β-carotene, and occurred in average proportions of 85, 11 and 4%, respectively, in fresh samples.	en_US
dc.identifier.citation	Food Chemistry Volume 55, Issue 1, 1996, Pages 63–72	en_US
dc.identifier.issn	0308-8146
dc.identifier.uri	http://ir-library.ku.ac.ke/handle/123456789/10003
dc.language.iso	en	en_US
dc.publisher	Elsevier	en_US
dc.title	An isocratic reversed-phase HPLC separation of the stereoisomers of the provitamin A carotenoids (α- and β-carotene) in dark green vegetables	en_US
dc.type	Article	en_US

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