Influence of Age and Previous Diet of Anopheles Gambiae on the Infectivity of Natural Plasmodium Falciparum Gametocytes from Human Volunteers.

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dc.contributor.authorOkech, Bernard A.
dc.contributor.authorGouagna, Louis C.
dc.contributor.authorKabiru, Ephantus W.
dc.contributor.authorBeier, John C.
dc.contributor.authorYan, Guiyun
dc.contributor.authorGithure, John I.
dc.date.accessioned2018-04-03T07:23:59Z
dc.date.available2018-04-03T07:23:59Z
dc.date.issued2004-10-22
dc.descriptionResearch Articleen_US
dc.description.abstractThe effect of age and dietary factors of Anopheles gambiae (Diptera: Culicidae) on the infectivity of natural Plasmodium falciparum parasites was studied. Mosquitoes of various ages (1–3, 4–7 and 8–11 day old) and those fed blood (either single or double meals) and sugar meals were experimentally co-infected with P. falciparum gametocytes obtained from different naturally infected human volunteers. On day 7, midguts were examined for oocyst infection to determine whether mosquito age or diets have significant effects on parasite infectivity. The age of the mosquitoes did not significantly influence the oocyst infection rates (χ2 = 48.32, df = 40, P = 0.172) or oocyst load (# of oocysts/midgut) (P = 0.14) observed. Oocyst load between groups was not significantly different. Similarly, the type of diet (either blood or sugar) did not influence oocyst infection rates (χ2 = 16.52, df = 19, P = 0.622). However, an increase in oocyst infection rates resulted after previous feeding on double blood meals (35%) compared to single blood meals (25%), with comparable oocyst load. These observations are in agreement with those reported in previous studies suggesting that increased mosquito nutritional reserves resulting from increased dietary resources is favorable for malaria infectivity. This field-based study indicates that vector competence of An. gambiae to natural P. falciparum parasites does not vary with age and that nutritional resources acquired prior to an infectious blood meal plays a crucial role in mosquito-parasite relationships.en_US
dc.description.sponsorshipNIH grants U19AI45511, D43TWO1143 and D43TW00920 and ICIPE African Regional Postgraduate Programme in Insect Science (ARPPIS) training fellowshipen_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/18258
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.subjectAn. gambiaeen_US
dc.subjectblood and sugar feedingen_US
dc.subjectmalaria infectivtyen_US
dc.subjectmosquito ageen_US
dc.subjectoocystsen_US
dc.subjectP. falciparumen_US
dc.subjectsporogonyen_US
dc.titleInfluence of Age and Previous Diet of Anopheles Gambiae on the Infectivity of Natural Plasmodium Falciparum Gametocytes from Human Volunteers.en_US
dc.typeArticleen_US
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