Genetic diversity and population structure of the human malaria parasite Plasmodium falciparum surface protein Pfs47 in isolates from the lowlands in Western Kenya

Simple item page
dc.contributor.authorOnyango, Shirley A.
dc.contributor.authorOchwedo, Kevin O.
dc.contributor.authorMachani, Maxwell G.
dc.contributor.authorOmondi, Collince J.
dc.contributor.authorDebrah, Isaiah
dc.contributor.authorOgolla, Sidney O.
dc.contributor.authorMing-Chieh, Lee
dc.contributor.authorGuofa, Zhou
dc.contributor.authorKokwaro, Elizabeth
dc.contributor.authorKazura, James W.
dc.contributor.authorAfrane, Yaw A.
dc.contributor.authorGitheko, Andrew K.
dc.contributor.authorDaibin, Zhong
dc.contributor.authorGuiyun, Yan
dc.date.accessioned2024-10-09T13:59:06Z
dc.date.available2024-10-09T13:59:06Z
dc.date.issued2021-11-29
dc.descriptionJournal Article
dc.description.abstractPlasmodium falciparum parasites have evolved genetic adaptations to overcome immune responses mounted by diverse Anopheles vectors hindering malaria control efforts. Plasmo dium falciparum surface protein Pfs47 is critical in the parasite’s survival by manipulating the vector’s immune system hence a promising target for blocking transmission in the mos quito. This study aimed to examine the genetic diversity, haplotype distribution, and popula tion structure of Pfs47 and its implications on malaria infections in endemic lowlands in Western Kenya. Cross-sectional mass blood screening was conducted in malaria endemic regions in the lowlands of Western Kenya: Homa Bay, Kombewa, and Chulaimbo. Dried blood spots and slide smears were simultaneously collected in 2018 and 2019. DNA was extracted using Chelex method from microscopic Plasmodium falciparum positive samples and used to genotype Pfs47 using polymerase chain reaction (PCR) and DNA sequencing. Thirteen observed haplotypes of the Pfs47 gene were circulating in Western Kenya. Popula tion-wise, haplotype diversity ranged from 0.69 to 0.77 and the nucleotide diversity 0.10 to 0.12 across all sites. All the study sites displayed negative Tajima’s D values although not significant. However, the negative and significant Fu’s Fs statistical values were observed across all the study sites, suggesting population expansion or positive selection. Overall genetic differentiation index was not significant (FST = -0.00891, P > 0.05) among parasite populations. All Nm values revealed a considerable gene flow in these populations. These results could have important implications for the persistence of high levels of malaria trans mission and should be considered when designing potential targeted control interventions.
dc.identifier.citation: Onyango SA, Ochwedo KO, Machani MG, Omondi CJ, Debrah I, Ogolla SO, et al. (2021) Genetic diversity and population structure of the human malaria parasite Plasmodium falciparum surface protein Pfs47 in isolates from the lowlands in Western Kenya. PLoS ONE 16(11): e0260434.
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0260434
dc.identifier.urihttps://ir-library.ku.ac.ke/handle/123456789/29127
dc.language.isoen
dc.publisherPLOS ONE
dc.titleGenetic diversity and population structure of the human malaria parasite Plasmodium falciparum surface protein Pfs47 in isolates from the lowlands in Western Kenya
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Full-text journal article.pdf
Size:
1.39 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Zoological Sciences