Promotion of Neuronal Differentiation Through Activation of N-Methyl-D-Aspartate Receptors Transiently Expressed by Undifferentiated Neural Progenitor Cells in Fetal Rat Neocortex

Simple item page
dc.contributor.authorYoneyama, Masanori
dc.contributor.authorNakamichi, Noritaka
dc.contributor.authorFukui, Masaki
dc.contributor.authorKitayama, Tomoya
dc.contributor.authorGeorgiev, Danko D.
dc.contributor.authorMakanga, Juliet O.
dc.contributor.authorNakamura, Nobuhiro
dc.contributor.authorTaniura, Hideo
dc.contributor.authorYoneda, Yukio
dc.date.accessioned2023-03-31T10:04:05Z
dc.date.available2023-03-31T10:04:05Z
dc.date.issued2008
dc.descriptionAbstracten_US
dc.description.abstractNeural progenitor cell is a generic term for undifferentiated cell populations composed of neural stem, neuronal progenitor, and glial progenitor cells with abilities for self-renewal and multipotentiality. In this study, we have attempted to evaluate the possible functional expression of N-methyl-D-aspartate (NMDA) receptors by neural progenitor cells prepared from neocortex of 18-day-old embryonic rats. Cells were cultured in the presence of basic fibroblast growth factor (bFGF) for different periods up to 12 days under floating conditions. Reverse transcription-polymerase chain reaction and fluorescence imaging analyses revealed transient expression of functional NMDA receptors in neurospheres formed by clustered progenitors during the culture with bFGF. A similarly potent increase was seen in the fluorescence intensity after brief exposure to NMDA in cells differentiated after the removal of bFGF under adherent conditions, and an NMDA receptor antagonist invariably prevented these increases by NMDA. Moreover, sustained exposure to NMDA not only inhibited the formation of neurospheres when exposed for 10 days from day 2 to day 12 but also promoted spontaneous and induced differentiation of neurospheres to cells immunoreactive for a neuronal marker protein on immunocytochemistry and Western blotting analyses. These results suggest that functional NMDA receptors may be transiently expressed to play a role in mechanisms underlying the modulation of proliferation along with the determination of subsequent differentiation fate toward a neuronal lineage in neural progenitor cells of developing raten_US
dc.description.sponsorshipMinistry of Education, Culture, Sports, Scienceand Technology, Japanen_US
dc.identifier.citationYoneyama, M., Nakamichi, N., Fukui, M., Kitayama, T., Georgiev, D. D., Makanga, J. O., ... & Yoneda, Y. (2008). Promotion of neuronal differentiation through activation of N‐methyl‐D‐aspartate receptors transiently expressed by undifferentiated neural progenitor cells in fetal rat neocortex. Journal of neuroscience research, 86(11), 2392-2402.en_US
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/jnr.21696
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/25075
dc.language.isoenen_US
dc.publisherWiley Library Onlineen_US
dc.subjectneural progenitor cellsen_US
dc.subjectneurospheresen_US
dc.subjectNMDA receptorsen_US
dc.subjectcommitmenten_US
dc.subjectdifferentiationen_US
dc.titlePromotion of Neuronal Differentiation Through Activation of N-Methyl-D-Aspartate Receptors Transiently Expressed by Undifferentiated Neural Progenitor Cells in Fetal Rat Neocortexen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Promotion of neuronal differentiation through activation.....pdf
Size:
152.67 KB
Format:
Adobe Portable Document Format
Description:
Abstract
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Pharmacy & Complementary / Alternative Medicine