Effects of glucocorticoids in Leishmania major infection

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dc.contributor.authorOsero, B. O.
dc.contributor.authorMosigisi, A.
dc.contributor.authorOgeto, T. K.
dc.contributor.authorMugambi, R.
dc.contributor.authorIngonga, J.
dc.contributor.authorKaranja, R. M.
dc.contributor.authorGicheru, M. M.
dc.contributor.authorAnjili, C.
dc.date.accessioned2016-07-12T08:36:23Z
dc.date.available2016-07-12T08:36:23Z
dc.date.issued2015
dc.descriptionResearch Articleen_US
dc.description.abstractLeishmania parasites activate NF-κB which induces Th2 expression and inactivates Th1 genes thus subverting the host defense response and promotes the survival and development of the parasite in macrophages. Macrophages were treated artificially with glucocorticoids and incubated with Leishmania promastigotes. Interleukin 1 β, Tumor necrosis factor- α and inhibitory nitric oxide synthase gene levels were measured using real time PCR and parasite development monitored in vitro. Tumor necrosis factor- α and nitric oxide synthase genes were down-regulated and Interleukin 1 β upregulated in macrophages treated with dexamethasone and hydrocortisone drugs when compared to those treated with lipopolysaccharide and untreated. Dexamethasone treated macrophages had significantly low number of amastigotes compared to hydrocortisone and lipopolysaccharide (p=0.0006). Dexamethasone showed high reduction of infection rates in macrophages as compared to hydrocortisone and lipopolysaccharide treated macrophages, however not significant (p=0.054). With further clinical studies in humans, dexamethasone may be used in the control of leishmaniasis. Keywords: Glucocorticoids, NF-kB, infection rates, amastigotes, macrophagesen_US
dc.identifier.citationInternational Journal of Fauna and Biological Studies, 2015en_US
dc.identifier.issn23472677
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/14828
dc.language.isoenen_US
dc.subjectGlucocorticoidsen_US
dc.subjectNF-kBen_US
dc.subjectInfection ratesen_US
dc.subjectAmastigotesen_US
dc.subjectMacrophagesen_US
dc.titleEffects of glucocorticoids in Leishmania major infectionen_US
dc.typeArticleen_US
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