Phylogenetic relationship and divergence among planktonic strains of Arthrospira (Oscillatoriales, Cyanobacteria) of African, Asian and American origin deduced by 16S–23S ITS and phycocyanin operon sequences

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dc.contributor.authorKotut, Kiplagat
dc.contributor.authorDadheech, P. K.
dc.contributor.authorBallot, A.
dc.contributor.authorCasper, P.
dc.contributor.authorNovelo, E.
dc.contributor.authorLemma, B.
dc.contributor.authorPröschold, T.
dc.contributor.authorKrienitz, L.
dc.date.accessioned2013-12-05T12:38:23Z
dc.date.available2013-12-05T12:38:23Z
dc.date.issued2010-07
dc.descriptionDOI: 10.2216/09-71.1en_US
dc.description.abstractArthrospira comprises multicellular, cylindrical, usually screwlike coiled trichomes and is cultivated commercially. In this study, 33 new strains of Arthrospira isolated from plankton samples collected in Mexico, East Africa and India were investigated and compared with 53 strains or samples of earlier considerations. The study included observations of morphological features and molecular phylogenetic analyses on the basis of nucleotide sequences of internal transcribed spacer (ITS) between 16S rRNA and 23S rRNA genes and partial sequences of beta and alpha subunits including intergenic spacer (cpcBA-IGS) of phycocyanin operon. Morphological traits of Arthrospira such as trichome width, type of coiling and apical cell were not always consistent in culture conditions. It was revealed that Arthrospira phylogeny on the basis of cpcBA-IGS locus was broadly comparable with the ITS region as both phylogenetic trees derived from nucleotide sequences could be divided into two main clusters. Cluster I comprised sequences from American strains mainly, whereas cluster II contained the sequences of the strains originating from Africa and Asia chiefly. Both genetic regions of the strains investigated in the present study coincidently showed a significant sequence divergence among Arthrospira strains from East Africa, India and Mexico indicating possible distinct evolutionary lineages. Keywords: Cyanobacteria, Oscillatoriales, Arthrospira, Phylogeny, Phycocyanin operon, ITS, Mexico, Africa, Indiaen_US
dc.identifier.citationPhycologia: July 2010, Vol. 49, No. 4, pp. 361-372.en_US
dc.identifier.issn0031-8884
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/7820
dc.language.isoenen_US
dc.publisherPhycologiaen_US
dc.subjectCyanobacteriaen_US
dc.subjectOscillatorialesen_US
dc.subjectArthrospiraen_US
dc.subjectPhylogenyen_US
dc.subjectPhycocyanin operonen_US
dc.subjectITSen_US
dc.subjectMexicoen_US
dc.subjectAfricaen_US
dc.subjectIndiaen_US
dc.titlePhylogenetic relationship and divergence among planktonic strains of Arthrospira (Oscillatoriales, Cyanobacteria) of African, Asian and American origin deduced by 16S–23S ITS and phycocyanin operon sequencesen_US
dc.typeArticleen_US
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