Molecular characterization and genotype distribution of thioester-containing protein 1 gene in Anopheles gambiae mosquitoes in western Kenya
| dc.contributor.author | Onyango, Shirley A. | |
| dc.contributor.author | Ochwedo, Kevin O. | |
| dc.contributor.author | Machani, Maxwell G. | |
| dc.contributor.author | Olumeh, Julius O. | |
| dc.contributor.author | Debrah, Isaiah | |
| dc.contributor.author | Omondi, Collince J. | |
| dc.contributor.author | Ogolla, Sidney O. | |
| dc.contributor.author | Ming Chieh, Lee | |
| dc.contributor.author | Guofa, Zhou | |
| dc.contributor.author | Kokwaro, Elizabeth | |
| dc.contributor.author | Kazura, James W. | |
| dc.contributor.author | Afrane, Yaw A. | |
| dc.contributor.author | Githeko, Andrew K. | |
| dc.contributor.author | Zhong, Daibin | |
| dc.contributor.author | Yan, Guiyun | |
| dc.date.accessioned | 2024-10-11T06:28:30Z | |
| dc.date.available | 2024-10-11T06:28:30Z | |
| dc.date.issued | 2022 | |
| dc.description | Journal Article | |
| dc.description.abstract | Background: Evolutionary pressures lead to the selection of efcient malaria vectors either resistant or susceptible to Plasmodium parasites. These forces may favour the introduction of species genotypes that adapt to new breeding habitats, potentially having an impact on malaria transmission. Thioester-containing protein 1 (TEP1) of Anopheles gambiae complex plays an important role in innate immune defenses against parasites. This study aims to character ize the distribution pattern of TEP1 polymorphisms among populations of An. gambiae sensu lato (s.l.) in western Kenya. Methods: Anopheles gambiae adult and larvae were collected using pyrethrum spray catches (PSC) and plastic dippers respectively from Homa Bay, Kakamega, Bungoma, and Kisumu counties between 2017 and 2020. Collected adults and larvae reared to the adult stage were morphologically identifed and then identifed to sibling species by PCR. TEP1 alleles were determined in 627 anopheles mosquitoes using restriction fragment length polymorphisms polymerase chain reaction (RFLP-PCR) and to validate the TEP1 genotyping results, a representative sample of the alleles was sequenced. Results: Two TEP1 alleles (TEP1*S1 and TEP1*R2) and three corresponding genotypes (*S1/S1, *R2/S1, and *R2/R2) were identifed. TEP1*S1 and TEP1*R2 with their corresponding genotypes, homozygous *S1/S1 and heterozygous *R2/S1 were widely distributed across all sites with allele frequencies of approximately 80% and 20%, respectively both in Anopheles gambiae and Anopheles arabiensis. There was no signifcant diference detected among the popula tions and between the two mosquito species in TEP1 allele frequency and genotype frequency. The overall low levels in population structure (FST=0.019) across all sites corresponded to an efective migration index (Nm=12.571) and low Nei’s genetic distance values (<0.500) among the subpopulation. The comparative fxation index values revealed minimal genetic diferentiation between species and high levels of gene fow among populations. Conclusion: Genotyping TEP1 has identifed two common TEP1 alleles (TEP1*S1 and TEP1*R2) and three corre sponding genotypes (*S1/S1, *R2/S1, and *R2/R2) in An. gambiae s.l. The TEP1 allele genetic diversity and population structure are low in western Kenya. | |
| dc.identifier.citation | Onyango, S. A., Ochwedo, K. O., Machani, M. G., Olumeh, J. O., Debrah, I., Omondi, C. J., ... & Yan, G. (2022). Molecular characterization and genotype distribution of thioester-containing protein 1 gene in Anopheles gambiae mosquitoes in western Kenya. Malaria journal, 21(1), 235. | |
| dc.identifier.uri | https://doi.org/10.1186/s12936-022-04256-w | |
| dc.identifier.uri | https://ir-library.ku.ac.ke/handle/123456789/29128 | |
| dc.language.iso | en | |
| dc.publisher | Malaria Journal | |
| dc.title | Molecular characterization and genotype distribution of thioester-containing protein 1 gene in Anopheles gambiae mosquitoes in western Kenya | |
| dc.type | Article |