Somatic Embryogenesis and Plant Regeneration of Farmer-Preferred Passion Fruit Varieties Grown in Kenya
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Date
2023-12
Authors
Asande, Lydia K.
Omwoyo, Richard O.
Oduor, Richard O.
Nyaboga, Evans N.
Journal Title
Journal ISSN
Volume Title
Publisher
mdip
Abstract
In vitro regeneration of passion fruit has great prospects for mass production of diseasefree planting materials. The objective of this study was to develop an in vitro regeneration system
through somatic embryogenesis for farmer-preferred genotypes grown in Kenya. Callus induction
and somatic embryogenesis were carried out using leaf and immature seed explants. The explants
were cultured on Murashige and Skoog medium augmented with different concentrations of 2,4-
dichlorophenoxyacetic acid (2,4-D) alone and 2,4-D combined with 1.0 mg L−1
thidiazuron (TDZ).
Data were recorded and subjected to analysis of variance. The highest number of somatic embryos
was obtained from KPF4 genotype using MS medium supplemented with 8 mg L−1 2,4-D and
1 mg L−1 TDZ. The embryos were converted to plants on germination medium comprising of MS
augmented with 0.5 mg L−1 6-benzyl amino purine (BAP). The plantlets were hardened for 4 weeks
in plastic pots. The survival rate during hardening of in vitro regenerated plants was 77.8%. The
present study reports a regeneration system through somatic embryogenesis for KPF4 passion fruit
grown in Kenya. The in vitro regeneration system can be utilized for mass propagation and genetic
improvement of KPF4 variety grown in Kenya.
Description
Article
Keywords
passion fruit, somatic embryogenesis, callus, cytokinins, auxins
Citation
Asande, L.K.; Ombori, O.; Oduor, R.O.; Nyaboga, E.N. Somatic Embryogenesis and Plant Regeneration of Farmer-Preferred Passion Fruit Varieties Grown in Kenya. Int. J. Plant Biol. 2023, 14, 1180–1189. https://doi.org/10.3390/ ijpb14040086