Characterization of occult hepatitis B virus infection among HIV positive patients in Cameroon

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dc.contributor.authorGachara, George
dc.contributor.authorMagoro, Tshifhiwa
dc.contributor.authorMavhandu, Lufuno
dc.contributor.authorLum, Emmaculate
dc.contributor.authorKimbi, Helen K.
dc.contributor.authorNdip, Roland N.
dc.contributor.authorBessong, Pascal O.
dc.date.accessioned2018-04-12T06:26:24Z
dc.date.available2018-04-12T06:26:24Z
dc.date.issued2017
dc.descriptionDOI 10.1186/s12981-017-0136-0en_US
dc.description.abstractPurpose: Occult hepatitis B infection (OBI) among HIV positive patients varies widely in different geographic regions. We undertook a study to determine the prevalence of occult hepatitis B infection among HIV infected individuals visiting a health facility in South West Cameroon and characterized occult HBV strains based on sequence analyses. Methods: Plasma samples (n = 337), which previously tested negative for hepatitis B surface antigen (HBsAg), were screened for antibodies against hepatitis B core (anti-HBc) and surface (anti-HBs) antigens followed by DNA extraction. A 366 bp region covering the overlapping surface/polymerase gene of HBV was then amplified in a nested PCR and the amplicons sequenced using Sanger sequencing. The resulting sequences were then analyzed for genotypes and for escape and drug resistance mutations. Results: Twenty samples were HBV DNA positive and were classified as OBI giving a prevalence of 5.9%. Out of these, 9 (45%) were anti-HBs positive, while 10 (52.6%) were anti-HBc positive. Additionally, 2 had dual anti-HBs and anti-HBc reactivity, while 6 had no detectable HBV antibodies. Out of the ten samples that were successfully sequenced, nine were classified as genotype E and one as genotype A. Three sequences possessed mutations associated with lamivudine resistance. We detected a number of mutations within the major hydrophilic region of the surface gene where most immune escape mutations occur. Conclusions: Findings from this study show the presence of hepatitis B in patients without any of the HBV serological markers. Further prospective studies are required to determine the risk factors and markers of OBI. Keywords: Hepatitis B virus, Occult hepatitis B infection, HIV, Cameroonen_US
dc.identifier.citationAIDS Research and Therapy (2017),14:11. DOI 10.1186/s12981-017-0136-0en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/18306
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectHepatitis B virusen_US
dc.subjectOccult hepatitis B infectionen_US
dc.subjectHIVen_US
dc.subjectCameroonen_US
dc.titleCharacterization of occult hepatitis B virus infection among HIV positive patients in Cameroonen_US
dc.typeArticleen_US
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