Experimental phage therapy against haematogenous multi-drug resistant Staphylococcus aureus pneumonia in mice

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dc.contributor.authorOduor, Joseph M. Ochieng’
dc.contributor.authorOnkoba, Nyamongo
dc.contributor.authorMaloba, Fredrick
dc.contributor.authorNyachieo, Atunga
dc.date.accessioned2017-03-07T07:29:34Z
dc.date.available2017-03-07T07:29:34Z
dc.date.issued2016
dc.descriptionResearch Paperen_US
dc.description.abstractBackground: Community-acquired haematogenous Staphylococcus aureus pneumonia is a rare infection, though it can be acquired nosocomially. Currently, antibiotics used against S. aureus pneumonia have shown reduced efficacy. Thus, there is need for an alternative therapy against multidrug-resistant S. aureus (MDRSA) strains in the community. Objective: We sought to determine the efficacy of environmentally-obtained S. aureus lytic phage against haematogenous MDRSA pneumonia in mice. Methods: Phages and MDRSA were isolated from sewage samples collected within Nairobi County, Kenya. Isolated S. aureus bacteria were screened for resistance against ceftazidime, oxacillin, vancomycin, netilmicin, gentamicin, erythromycin, trimethroprim-sulfamethoxazole and cefuroxime. Thirty BALB/c mice aged six to eight weeks were randomly assigned into three groups: the MDRSA-infection group (n = 20), the phage-infection group (n = 5) and the non-infection group (n = 5). Mice were infected with either MDRSA or phage (108 CFU/mL) and treated after 72 hours with a single dose of clindamycin (8 mg/kg/bwt) or 108 PFU/mL of phage or a combination therapy (clindamycin and phage). The efficacy of phage, clindamycin or clindamycin with phage combination was determined using resolution of lung pathology and bacterial load in lung homogenates. Results: The viable MDRSA count was 0.5 ± 0.2 log10 CFU/gm in the phage-treated group, 4.4 ± 0.2 log10 CFU/gm in the clindamycin-treated group and 4.0 ± 0.2 log10 CFU/gm in the combination-treated group. The efficacy of phage therapy was significantly different from other therapeutic modes (p = 0 < 0.0001). Histology showed that the mice treated with phage did not develop pneumonia. Conclusion: Phage therapy is effective against haematogenous MDRSA infection. Thus, it can be explored as an alternative treatment method.en_US
dc.description.sponsorshipInstitute of Primate Researchen_US
dc.identifier.citationOchieng’ Oduor JM, Onkoba N, Maloba F, Nyachieo A. Experimental phage therapy against haematogenous multi-drug resistant Staphylococcus aureus pneumonia in mice. Afr J Lab Med. 2016;5(1), a435. http:// dx.doi.org/10.4102/ajlm. v5i1.435en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/15415
dc.language.isoenen_US
dc.publisherAOSISen_US
dc.titleExperimental phage therapy against haematogenous multi-drug resistant Staphylococcus aureus pneumonia in miceen_US
dc.typeArticleen_US
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