Cytokines Associated with Antiretroviral Induced Hepatotoxicity in People Infected With the Human Immunodeficiency Virus Type 1 in the Northwest Region of Cameroon
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Date
2018
Authors
Abongwa, Lem Edith
Nyamache, Anthony Kebira
Fokunang, Charles
Torimiro, Judith
Djukouo, Larissa
Ngu, Loveline
Okemo, Paul
Nchinda, Godwin
Journal Title
Journal ISSN
Volume Title
Publisher
IJMRPS
Abstract
The advent of highly active antiretroviral therapy (HAART) has enabled HIV-1 infected people to live long and fruitful lives. Since drug and viral mediated toxicities are hallmarked by a modulation of patient’s cytokine profiles, we assessed the impact of ART on plasma cytokine profiles of HIV-1 patients. Blood samples were collected from 68 HAART naïve HIV-1 patients at baseline (D0), 30 days (D30) and 180 days (D180) following HAART initiation. Serum levels of Alanine aminotransferases (ALT) and aspartate aminotransferases (AST) was analysis enzymatically.Human Th1/Th2/Th17 cytokines were measured using a cytometric bead array assay. Data were analyzed using Graph pad prism 6 and SSPS.There was a significant increased (p<0.001) in the mean serum levels of both ALT and AST corresponding with the treatment duration.A negative correlation was observed between CD4+ T cell counts and serum levels of either ALT (-0.522, p=0.000) or AST (-0.505, p=0.000). The prevalence of hepatotoxicity increased significantly (P =0.000) and was found to be 0(0.0%), 34(50.0%) and 47(69.1%) at D0, D30 and D180 respectively. Mean IL-2, IL-6, IL-17A and TNF-α cytokines were higher in patients with hepatotoxicity compared to patients with no hepatotoxicity at D30 and D180 with a significant difference (p<0.05) seen only in IL 17-A and IL 6. The prevalence of hepatotoxicity increased with treatment duration and was associated with modulations in the human Th1/Th2/Th17 cytokine profile. IL-6 and IL-17A seem to play a significant role in the pathophysiology of hepatotoxicity. As such they might be used either alone or with other biomarkers to assess HAART induced hepatotoxicity in our context.
Description
Research Article
Keywords
Cytokines, HAART, HIV-1, Hepatotoxicity, Alanine aminotransferases, Aspartate aminotransferases