Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates

Simple item page
dc.contributor.authorMburu, D. N.
dc.contributor.authorMisigo, D.
dc.contributor.authorMwaengo, D.
dc.date.accessioned2015-02-06T08:23:04Z
dc.date.available2015-02-06T08:23:04Z
dc.date.issued2014
dc.descriptiondoi: 10.3855/jidc.3050.en_US
dc.description.abstractIntroduction: The commonly expected causative agents associated with flu-like symptoms in Kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (RSV) and rhinovirus. However, newer agents have been identified globally that present with illnesses clinically indistinguishable from those caused by the classical pathogens; one of them is human bocavirus. Methodology: A total of 384 specimens were analyzed, primarily to determine if the emerging human bocavirus (HBoV) infections exist in Kenya as coinfections with other respiratory viruses and to describe the genotype of the virus in circulation. In brief, viral nucleic acids were extracted from culture supernatants, amplified by PCR, and sequenced. Results: HBoV DNA was amplified from 1.8% of screened specimens. Coinfection with parainfluenza virus, adenovirus, and enterovirus was 2.5%, 2%, and 1.4%, respectively. Multiple coinfections consisting of HBoV plus two other viruses were found in 3% of specimens. Isolation occurred in the months of January, March, April, August, and November. Retrospective review of clinical parameters indicated that all the individuals complained of non-specific symptoms, mainly fever, coughs, nasal stuffiness, runny noses, and vomiting. Phylogenetically, the GenBank deposited sequences of this study’s isolates cluster closely to the reference strain NC_07455 (HBoV1). Conclusion: Coinfections with human bocavirus (HBoV1) occur in Kenya, and high incidence might primarily be during the early stages of children’s livesen_US
dc.description.sponsorshipWe give special thanks to the late Dr Walter O. Ochieng for his support and permission to use his laboratory and resources at the National Influenza Center. We also extend our gratitude to Lucy Muthui and Ben Kiawa of the International Livestock Research Institute (ILRI) for procuring our primers and assisting with the sequencing. The study was conducted through our own contributions and partial resource (reagents/materials) support from the National Influenza Center of Kenya through the late Dr Walter Ochiengen_US
dc.identifier.citationJournal of infection in developing countries, 2014 Feb 13;8(2):221-7. doi: 10.3855/jidc.3050.en_US
dc.identifier.issn1972-2680
dc.identifier.issn2036-6590
dc.identifier.issn1972-2680
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/12150
dc.language.isoenen_US
dc.publisherOpen Learning on Enteric Pathogensen_US
dc.subjecthuman bocavirusen_US
dc.subjectKenya isolatesen_US
dc.subjectrespiratory viral infectionsen_US
dc.titleMolecular detection and phylogenetic analysis of Kenyan human bocavirus isolatesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Molecular detection and phylogenetic analysis of.....pdf
Size:
333.52 KB
Format:
Adobe Portable Document Format
Description:
Full text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Chemistry