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dc.contributor.authorMachuka, Jesse
dc.contributor.authorMatheka, J. M.
dc.contributor.authorMagiri, E.
dc.contributor.authorOmer, Rasha A
dc.date.accessioned2014-05-12T11:58:22Z
dc.date.available2014-05-12T11:58:22Z
dc.date.issued2014-05-12
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/9487
dc.description.abstractTolerance to salinity was obtained in an open pollinated variety (KAT) widely grown in the East African region and a dryland hybrid (PH01) by applying in vitro selection and regeneration procedures. Immature zygotic embryos of KAT and PH01 plants were cultured on N6 medium supplemented with 2mg/l 2,4-dichlorophenoxyacetic acid to initiate embryogenic calli. Calli were then maintained for one month after which time they were subjected to increasing concentrations of NaCl (between 0 and 2.9%) to determine the appropriate concentrations of selection pressure. The survival and regeneration capacity of KAT and PH01 calli were significantly lower (p<0.05) than those of their controls after exposure on both levels of NaCl. The genotype did not influence the survival capacity of selected calli. However, KAT and PH01 were found to differ significantly (p<0.05) in regeneration capacityen_US
dc.language.isoenen_US
dc.subjectSomaclonal variationen_US
dc.subjectMaizeen_US
dc.subjectSalinity toleranceen_US
dc.subjectRAPDen_US
dc.titleIn vitro selection and characterization of salinity tolerant somaclones of tropical maize (Zea mays L.)en_US
dc.typeOtheren_US


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