Transcriptional profiling of sorghum midge defense responses in sorghum using CDNA-AFLP
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Date
2014-03-24
Authors
Mbinda, Wilton Mwema
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Abstract
Sorghum midge (Stenodiplosis sorghicola (Coquillett), is the most ubiquitous damaging pest of
grain sorghum [Sorghum bicolor (L.) Moench] worldwide. In spite of its economic importance,
little is known about the molecular basis of compatible interaction between the pest and sorghum
host. Monitoring the transcriptome changes in sorghum in response to developing embryofeeding
sorghum midge will provide a solid foundation to understand the genetic mechanisms of
the plant defense against a sorghum midge attack. To identify genes that are differentially
expressed in sorghum upon sorghum midge attack, a transcriptome analysis using cDNA-AFLP
was profiled in the sorghum midge resistant and susceptible genotypes at time intervals 0, 3, 6
and 12 hours. In total, 12 different primer combinations were used, generating cDNA fragments
ranging from 50 bp to 500 bp in size. Of approximately 6,720 cDNA fragments generated, 118
were novel, 17 up-regulated and 23 were down regulated genes. These identified cDNAs were
cloned, sequenced, and aligned against GenBank databases using National Centre for
Biotechnology Information BlastX and BlastN tools. Database analysis predicted that the
proteins encoded by these cDNAs are involved in signal transduction, secondary metabolism,
oxidative response, photosynthesis, cell maintenance, and other cellular activities. There are also
a large number of identified genes with unknown function. Of the 53 reliable cDNA-AFLP
transcripts, 4 homologous to genes known to play a role in defense genes were further selected
for validation of cDNA-AFLP expression patterns using qRT-PCR analyses. Overall, the profiles
of sorghum genes responsive to sorghum midge feeding indicated that defense genes in sorghum
were co-ordinately regulated. Collectively, the results suggest that the transcripts differentially
expressed during sorghum midge feeding represent candidate genes of sorghum defense response
to sorghum midge stress. Maize protease inhibitor, subtilisin/chymotrypsin inhibitor CI-IB, and
cinnamoyl-CoA reductase 2 proteins were hypothesized to be vital for enabling the plant to
overcome the stresses inflicted by sorghum midge feeding. The results show that cDNA-AFLP is
a reliable technique for studying expression patterns of genes involved in the sorghum-sorghum
midge interactions. This study provides the first global catalogue of genes expressed during
sorghum and sorghum midge infestation, together with their functional annotations. The results
will help to elucidate the molecular basis of the attack process and identify genes and chemicals
that could help to inhibit the growth of the pest.
Description
Department of Biochemistry and Biotechnology, 93p. The SB 608 .S6M82 2010
Keywords
Sorghum --Diseases and pests, Sorghum Midge, Sorghum root and stalk rots