Efficacy of Cryptolepis sanguinolenta root extract on slow-growing rifampicin resistant Mycobacterium tuberculosis
Loading...
Date
2012-02
Authors
Kirimuhuzya, Claude
Bunalema, Lydia
Waako, Paul
Tabuti, John R. S.
Orodho, John Aluko
Magadula, Jangu Joseph
Otieno, Nicholas
Okemo, P. O.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
We report here, the results from total crude methanol extract as well as serial ether, chloroform, and
methanol extracts of the root of Cryptolepis sanguinolenta (Lindl) Schltr that were screened against
three strains of Mycobacterium tuberculosis. The strains used included the pan sensitive H37Rv, the
rifampicin-resistant TMC-331 and a wild strain of Mycobacetrium avium (MA) isolated from a Ugandan
patient. The disc diffusion method was used for susceptibility tests on solid Middle brook 7H10 while
the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were
determined by the microtitre plate method using Middle brook 7H9 broth. We report that the total crude
methanol extract showed the highest activity against H37Rv and TM-331 with complete clearance of
quadrants at 50 mg/ml and zones of inhibition of 10.0 to 11 mm at 25 mg/ml concentration although it
was not effective against M. avium. The corresponding MIC values were 1.17 mg/ml for H37Rv and 1.56
mg/ml for TMC-331. The values for isoniazid were 0.25 and 9.38 µg/ml for H37Rv and TMC-331,
respectively, while for rifampicin the MIC value was 0.25 µg/ml for H37Rv but it was not active on TMC-
331. Acute toxicity test gave an LD50 of 758.5 mg/kg body weight while the phytochemical analysis
showed the presence of alkaloids, tannins and flavonoids.
Description
Keywords
Anti-mycobacterial, in vitro activity, medicinal plant, Cryptolepis sanguinolenta, Mycobacterium tuberculosis, Mycobacterium avium, rifampicin, isoniazid
Citation
Journal of Medicinal Plants Research Vol. 6(7), pp. 1140-1146, 23 February, 2012