Efficacy of 13 medicinal plants used by indigenous communities around Lake Victoria, Kenya, against tuberculosis, diarrhoea aausing bacteria and candida albicans
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Date
2010-09
Authors
Mariita, Richard M.
Okemo, P. O.
Orodho, John Aluko
Kirimuhuzya, Claude
Otieno, Joseph N.
Magadula, J. Joseph
Journal Title
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Volume Title
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Abstract
Aims: To investigate Crude methanol extracts of 13 medicinal plants obtained through an ethnobotanical survey
against 4 strains of mycobacteria (Mycobacterium tuberculosis, M. kansasii, M. fortuitum and M. smegmatis),
Salmonella typhi, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia and
Candida albicans.
Materials and Methods: Antimycobacterial susceptibility tests were carried out using BACTEC MGIT 960
system. Evaluation of antibacterial, antifungal and phytochemical properties was done using standard
procedures.
Results and Discussion: All the plant extracts inhibited mycobacterial growth at 2.0 mg/mL. Carissa edulis and
Vernonia amygdalina were the most potent against M. smegmatis and M. fortuitum, completely inhibiting their
Mariita *et al. /International Journal Of Pharmacy&Technology
IJPT | Sep-2010 | Vol. 2 | Issue No.3 | 771-791 Page 772
growth (Zero GUs) at all concentrations used. Toddalia asiatica had high inhibitory activity (Zero GUs) against
M. tuberculosis and M. kansasii at all concentrations used. There was a significant difference on general
antibacterial results of the extracts at P≤0.05 against other test cultures. The most potent antibacterial extract was
from Toddalia asiatica with an MIC and MBC of 9.375 mg/mL. Carissa edulis and Momordica charantia both
produced MICs and MBCs of 37.5 mg/mL against S. typhi and S. aureus. Lantana camara produced MICs and
MBCs of 37.5 mg/mL against both S. aureus and P. aeruginosa. Preliminary phytochemistry identified six
phytochemicals with flavonoids being found in all extracts.
Conclusion: The data suggests that methanolic extracts of some of the plant species can be used against several
microbial agents. Further work on them is underway.
Description
Available at www.ijptonline.com/wp-content/uploads/2009/.../771-791.pdf
Keywords
Antimicrobial, Diarrhoeal, Lake Victoria region, Medicinal plants, Tuberculosis, phytochemicals
Citation
International Journal Of Pharmacy&Technology Sep-2010 | Vol. 2 | Issue No.3 | 771-791