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dc.contributor.advisorKenya, E.U.
dc.contributor.advisorOsir, E. O.
dc.contributor.advisorAbubakar, L. U.
dc.contributor.authorBurugu, Marion Warigia
dc.date.accessioned2012-10-19T12:25:06Z
dc.date.available2012-10-19T12:25:06Z
dc.date.issued2012-10-19
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/5771
dc.descriptionBiochemistry and Biotechnology Department, 70p. The SF 967.T78B8 2004en_US
dc.description.abstractAfrican animal trypanosomosis, transmitted by the tsetse fly remains a major constraint to food security in areas of Africa that hold the continent's greatest potential for expanded agriculture production. The flies also transmit the parasite responsible for human sleeping sickness. Other haematophagous arthropods like stable flies also transmit trypanosomes mechanically. Mechanical transmission only accounts for a small percentage (~16 %) of the trypanosomes transmission since the haematophagous arthropods do not provide an environment for trypanosome development within their tissues unlike in tsetse flies. The life cycle of the parasitic African trypanosome in its invertebrate vector begins when the tsetse fly feeds from an infected mammalian host. Within the fly, the parasites are transformed from the bloodstream forms to procyclic forms and an infection is established. Many midgut enzymes have been shown to play a role in the establishment phase of the infection. Lectin-trypsin complex is one of the molecules that have been shown to play a role in the transformation of trypanosomes from bloodstream forms to procyclic forms vitro, the first crucial step in the establishment of primary midgut infections in the fly. proteolytic lectin gene encodes the a subunit of the lectin-trypsin protein. A study was undertaken to evaluate the expression profile of in fuscipes fuscipes using RT- PCR based techniques. In teneral flies, very low levels of transcript could be detected, but the expression was rapidly induced as early as 1 h post bloodmeal acquisition. Transcript levels decreased slightly after 6 h and stabilized, then increased after 24 h and peaked between 48 and 72 h of starvation. A further decrease of transcript levels was observed. The transcript abundance after 120h of starvation was higher than in teneral guts. This non-constitutive mode of expression suggests that the gene is regulated at the transcription level. Analysis for the expression of Gpl was also carried out in and Siomoxys calcirans besides the known cyclic vectors of trypanosomes using RT-PCR technique. The evaluation of proteolytic lectin gene in these haematophagous arthropods by PCR revealed that this gene is only expressed in Glossina species. The conservation of this gene in Glossina species only may be the reason for the cyclic mode of transmission of trypanosomes exhibited by tsetse flies.en_US
dc.description.sponsorshipKenyatta Universityen_US
dc.language.isoenen_US
dc.subjectTrypanosomiasis in cattleen_US
dc.subjectCattle--Infections
dc.titleExpression of the glossina proteolytic lectin (gpl) gene in flies and other haematophagous arthropodsen_US
dc.typeThesisen_US


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