Isolation and Antiproliferative Characterisation of Bergenin from Root Extract of Uvariodendron Anisatum Verdec (Annonaceae)
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Date
2019
Authors
Onyancha, J. M.
Gikonyo, N. K.
Wachira, S. W.
Gicheru, M. M.
Journal Title
Journal ISSN
Volume Title
Publisher
Drug Discovery
Abstract
Uvariodendron anisatum (annonaceae) is a rare, endemic and indigenous medicinal plant in Kenya. The plant root decoction is used
to remove placenta in women if it is retained during child deliveries. It is also known to management of impotency in men. Despite
this folkloric claim, information about phytochemical composition and biological activities are needed. The objective of this study
ANALYSIS Vol.13, 2019
DRUG DISCOVERY
ISSN
2278–540X
EISSN
2278–5396
Page55
© 2019 Discovery Publication. All Rights Reserved. www.discoveryjournals.org OPEN ACCESS
ARTICLE ANALYSIS
was to carryout isolation and antiproliferative characterisation of methanol extract of the whole root, fractions thereof and isolated
compound. Extract was prepared by maceration using methanol. Fractions of petroleum ether, dichloromethane, ethylacetate,
acetone and methanol were prepared by column chromatography. The compound was achieved by column and thin layer
chromatography and structural elucidation was done using spectral data and comparison with published literature. Methyl thiazole
tetrazolium (MTT) assay was used to study the effects of the extract, fractions and isolated compound against cancers of the
prostate and breast (DU-145 and HCC 1395 respectively). Vero cells were used as a control celline. The isolated compound was
identified as anhydrous bergenin. The extract revealed high antiproliferative effects against normal cell line (CC50 = 3.3±0.2µg/ml)
whereas it was moderately antiproliferative against prostate and breast cancer cell lines (IC50 =.81.7±3.9 and 50.6±2.9µg/ml)
respectively. Polar fractions revealed weak antiproliferative effects whereas the non polar fractions and the isolated compounds did
not reveal antiproliferative effects against cancer and normal cell lines. It was concluded that the extract of U. Anisatum was highly
antiproliferative against normal cell line but moderately cytotoxic against prostate and breats cancer cell lines. The antiproliferaive
effects were not attributed to bergenin and it was recommended that continued isolation and bioassay studies are necessary to
determine the compounds that are responsible for antiproliferative effects.
Description
Keywords
Annonaceae, Cell lines, Chromatography, Fractionation, Kenya, Medicinal plants, Spectroscopy
Citation
Onyancha JM, Gikonyo NK, Wachira SW, Gicheru MM. Isolation and antiproliferative characterisation of bergenin from root extract of Uvariodendron anisatum Verdec (Annonaceae). Drug Discovery, 2019, 13, 55-78